Phenotype and severity of asthma determines bronchial epithelial immune responses to a viral mimic.
Eur Respir J
; 60(1)2022 07.
Article
en En
| MEDLINE
| ID: mdl-34916261
ABSTRACT
BACKGROUND:
Asthma is characterised by an aggravated immune response to respiratory viral infections. This phenomenon is a clinically well-recognised driver of acute exacerbations, but how different phenotypes of asthma respond immunologically to viruses is unclear.OBJECTIVES:
To describe the association between different phenotypes and severity of asthma and bronchial epithelial immune responses to viral stimulation.METHODS:
In the Immunoreact study, healthy subjects (n=10) and 50 patients with asthma were included; 30 (60%) were atopic, and 34 (68%) were eosinophilic; 14 (28%) had severe asthma. All participants underwent bronchoscopy with collection of bronchial brushings. Bronchial epithelial cells (BECs) were expanded and stimulated with the viral replication mimic poly (IC) (Toll-like receptor (TLR)3 agonist) in vitro. The expression of TLR3-induced pro-inflammatory and antiviral responses of BECs were analysed using reverse transcriptase quantitative PCR and multiplex ELISA and compared across asthma phenotypes and severity of disease.RESULTS:
Patients with atopic asthma had increased induction of interleukin (IL)-4, interferon (IFN)-ß, IL-6, tumour necrosis factor-α, and IL-1ß after poly (IC) stimulation compared to non-atopic patients, whereas in patients with eosinophilic asthma only IL-6 and IL-8 induction was higher than in non-eosinophilic asthma. Patients with severe asthma displayed a decreased antiviral IFN-ß, and increased expression of IL-8, most pronounced in atopic and eosinophilic asthmatics. Furthermore, induction of IL-33 in response to poly (IC) was increased in severe atopic and in severe eosinophilic asthma, but thymic stromal lymphopoietin only in severe eosinophilic asthma.CONCLUSIONS:
The bronchial epithelial immune response to a viral mimic stimulation differs between asthma phenotypes and severities, which may be important to consider when targeting novel asthma treatments.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Asma
/
Interleucina-8
Límite:
Humans
Idioma:
En
Revista:
Eur Respir J
Año:
2022
Tipo del documento:
Article
País de afiliación:
Dinamarca