Cryo-EM analysis of Ebola virus nucleocapsid-like assembly.
STAR Protoc
; 3(1): 101030, 2022 03 18.
Article
en En
| MEDLINE
| ID: mdl-34977676
ABSTRACT
This protocol describes the reconstitution of the filamentous Ebola virus nucleocapsid-like assembly in vitro. This is followed by solving the cryo-EM structure using helical reconstruction, and flexible fitting of the existing model into the 5.8 Å cryo-EM map. The protocol can be applied to other filamentous viral protein assemblies, particularly those with high flexibility and moderate resolution maps, which present technical challenges to model building. For complete details on the use and execution of this profile, please refer to Su et al. (2018).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fiebre Hemorrágica Ebola
/
Ebolavirus
Límite:
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2022
Tipo del documento:
Article
País de afiliación:
China