Your browser doesn't support javascript.
loading
Target-induced silver nanocluster generation for highly sensitive electrochemical aptasensor towards cell-secreted interferon-γ.
Zhou, Yanli; Liu, Jiaxiang; Dong, Hui; Liu, Zi; Wang, Lu; Li, Qiaoxia; Ren, Jianhua; Zhang, Yintang; Xu, Maotian.
Afiliación
  • Zhou Y; Henan Key Laboratory of Biomolecular Recognition and Sensing, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000, PR China. Electronic address: zhouyanli@
  • Liu J; College of Environmental and Chemical Engineering, Shanghai University of Electric Power, Shanghai, 20090, PR China.
  • Dong H; Henan Key Laboratory of Biomolecular Recognition and Sensing, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000, PR China.
  • Liu Z; Henan Key Laboratory of Biomolecular Recognition and Sensing, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000, PR China.
  • Wang L; Henan Key Laboratory of Biomolecular Recognition and Sensing, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000, PR China.
  • Li Q; College of Environmental and Chemical Engineering, Shanghai University of Electric Power, Shanghai, 20090, PR China. Electronic address: liqiaoxia@shiep.edu.cn.
  • Ren J; College of Environmental and Chemical Engineering, Shanghai University of Electric Power, Shanghai, 20090, PR China.
  • Zhang Y; College of Environmental and Chemical Engineering, Shanghai University of Electric Power, Shanghai, 20090, PR China.
  • Xu M; Henan Key Laboratory of Biomolecular Recognition and Sensing, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000, PR China. Electronic address: xumaotian@
Biosens Bioelectron ; 203: 114042, 2022 May 01.
Article en En | MEDLINE | ID: mdl-35124342
Interferon-gamma (IFN-γ) is one kind of crucial inflammatory cytokines, and its expression level is closely associated with various disease progressions. This work addresses the development of a sensitive and specific electrochemical assay for detection of IFN-γ by combing the recognition unit of aptamer with the signal reporter of target-induced silver nanoclusters (AgNCs). For biosensor preparation, the gold nanoparticles (AuNPs) immobilized on the amine-terminated electrode surface provided electrochemical interfaces for the self-assembly of C-rich modified aptamers. Then, the aptamer recognized IFN-γ and the free aptamer hybridized with conjugated DNA sequences. After the nuclease-catalyzed cleavage of DNA duplex, in situ-generated AgNCs in the C-rich template was utilized as the electrochemical indicator for IFN-γ detection. The present method demonstrated a good performance for detection of IFN-γ with a low detection limit of 1.7 pg mL-1. This aptasensor was verified to be applied for the evaluation of IFN-γ secreted by cell.
Asunto(s)
Palabras clave
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Aptámeros de Nucleótidos / Nanopartículas del Metal Tipo de estudio: Diagnostic_studies Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2022 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Aptámeros de Nucleótidos / Nanopartículas del Metal Tipo de estudio: Diagnostic_studies Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2022 Tipo del documento: Article