Expression and Surface Display of an Acidic Cold-Active Chitosanase in <i>Pichia pastoris</i> Using Multi-Copy Expression and High-Density Cultivation.

Peng, Yanhong; Wang, Yaping; Liu, Xiaoyan; Zhou, Ronghua; Liao, Xianqing; Min, Yong; Ma, Lixin; Wang, Ying; Rao, Ben

Expression and Surface Display of an Acidic Cold-Active Chitosanase in Pichia pastoris Using Multi-Copy Expression and High-Density Cultivation.

Peng, Yanhong; Wang, Yaping; Liu, Xiaoyan; Zhou, Ronghua; Liao, Xianqing; Min, Yong; Ma, Lixin; Wang, Ying; Rao, Ben.

Afiliación

Peng Y; National Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Wang Y; State Key Laboratory of Biocatalysis and Enzyme, Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, Biology Faculty of Hubei University, Hubei University, Wuhan 430062, China.
Liu X; National Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Zhou R; State Key Laboratory of Biocatalysis and Enzyme, Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, Biology Faculty of Hubei University, Hubei University, Wuhan 430062, China.
Liao X; National Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Min Y; National Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Ma L; National Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Wang Y; National Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Rao B; State Key Laboratory of Biocatalysis and Enzyme, Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, Biology Faculty of Hubei University, Hubei University, Wuhan 430062, China.

Molecules ; 27(3)2022 Jan 26.

Article en En | MEDLINE | ID: mdl-35164064

Asunto(s)

Bacillus/genética; Proteínas Bacterianas/genética; Clonación Molecular; Glicósido Hidrolasas/genética; Pichia/genética; Bacillus/metabolismo; Proteínas Bacterianas/metabolismo; Frío; Estabilidad de Enzimas; Fermentación; Expresión Génica; Glicósido Hidrolasas/metabolismo; Pichia/metabolismo; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo

Palabras clave

Pichia pastoris; chiton oligosaccharides; chitosanase; displayed enzyme; high-density fermentation; multi-copy strains

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Pichia / Bacillus / Proteínas Bacterianas / Clonación Molecular / Glicósido Hidrolasas Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: China

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