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BAF complex-mediated chromatin relaxation is required for establishment of X chromosome inactivation.
Keniry, Andrew; Jansz, Natasha; Gearing, Linden J; Wanigasuriya, Iromi; Chen, Joseph; Nefzger, Christian M; Hickey, Peter F; Gouil, Quentin; Liu, Joy; Breslin, Kelsey A; Iminitoff, Megan; Beck, Tamara; Tapia Del Fierro, Andres; Whitehead, Lachlan; Jarratt, Andrew; Kinkel, Sarah A; Taberlay, Phillippa C; Willson, Tracy; Pakusch, Miha; Ritchie, Matthew E; Hilton, Douglas J; Polo, Jose M; Blewitt, Marnie E.
Afiliación
  • Keniry A; The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia. keniry@wehi.edu.au.
  • Jansz N; The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia. keniry@wehi.edu.au.
  • Gearing LJ; The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
  • Wanigasuriya I; The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.
  • Chen J; The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
  • Nefzger CM; The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.
  • Hickey PF; The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
  • Gouil Q; The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.
  • Liu J; Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC, Australia.
  • Breslin KA; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Clayton, VIC, Australia.
  • Iminitoff M; Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia.
  • Beck T; Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC, Australia.
  • Tapia Del Fierro A; The University of Queensland, Institute for Molecular Bioscience, St Lucia, QLD, Australia.
  • Whitehead L; The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
  • Jarratt A; The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.
  • Kinkel SA; The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
  • Taberlay PC; The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.
  • Willson T; The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
  • Pakusch M; The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.
  • Ritchie ME; The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
  • Hilton DJ; The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
  • Polo JM; The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.
  • Blewitt ME; The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
Nat Commun ; 13(1): 1658, 2022 03 29.
Article en En | MEDLINE | ID: mdl-35351876
ABSTRACT
The process of epigenetic silencing, while fundamentally important, is not yet completely understood. Here we report a replenishable female mouse embryonic stem cell (mESC) system, Xmas, that allows rapid assessment of X chromosome inactivation (XCI), the epigenetic silencing mechanism of one of the two X chromosomes that enables dosage compensation in female mammals. Through a targeted genetic screen in differentiating Xmas mESCs, we reveal that the BAF complex is required to create nucleosome-depleted regions at promoters on the inactive X chromosome during the earliest stages of establishment of XCI. Without this action gene silencing fails. Xmas mESCs provide a tractable model for screen-based approaches that enable the discovery of unknown facets of the female-specific process of XCI and epigenetic silencing more broadly.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Inactivación del Cromosoma X / ARN Largo no Codificante Límite: Animals Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2022 Tipo del documento: Article País de afiliación: Australia
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Inactivación del Cromosoma X / ARN Largo no Codificante Límite: Animals Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2022 Tipo del documento: Article País de afiliación: Australia