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Assessment of angiogenesis-related parameters in juvenile idiopathic arthritis-associated uveitis.
Baquet-Walscheid, Karoline; Wildschütz, Lena; Kasper, Maren; Busch, Martin; Thanos, Solon; Bauer, Dirk; Stoll, Monika; König, Simone; Heiligenhaus, Arnd.
Afiliación
  • Baquet-Walscheid K; Department of Ophthalmology and Ophtha-Lab, St. Franziskus-Hospital, Hohenzollernring 74, 48145, Münster, Germany. karoline.walscheid@uveitis-zentrum.de.
  • Wildschütz L; University of Duisburg-Essen, Essen, Germany. karoline.walscheid@uveitis-zentrum.de.
  • Kasper M; Department of Ophthalmology and Ophtha-Lab, St. Franziskus-Hospital, Hohenzollernring 74, 48145, Münster, Germany.
  • Busch M; Department of Ophthalmology and Ophtha-Lab, St. Franziskus-Hospital, Hohenzollernring 74, 48145, Münster, Germany.
  • Thanos S; Department of Ophthalmology and Ophtha-Lab, St. Franziskus-Hospital, Hohenzollernring 74, 48145, Münster, Germany.
  • Bauer D; Institute of Experimental Ophthalmology, University of Muenster, Münster, Germany.
  • Stoll M; Department of Ophthalmology and Ophtha-Lab, St. Franziskus-Hospital, Hohenzollernring 74, 48145, Münster, Germany.
  • König S; Core Facility Genomics, University of Muenster, Münster, Germany.
  • Heiligenhaus A; Department "Genetic Epidemiology", Institute of Human Genetics, University of Münster, Münster, Germany.
Mol Biol Rep ; 49(7): 6093-6102, 2022 Jul.
Article en En | MEDLINE | ID: mdl-35359237
PURPOSE: Juvenile idiopathic arthritis-associated uveitis (JIAU) may run a chronic and treatment-resistant course, and occasionally, alterations of the iris vasculature may be observed clinically. METHODS: Iris tissue (IT), aqueous humor (AH) and serum samples from patients with clinically inactive JIAU (n = 30), acute anterior uveitis (AAU; n = 18), and primary open angle glaucoma (POAG; n = 20) were obtained during trabeculectomy or cataract surgery. Samples were analyzed by RNA-Seq, qRT-PCR, LC-IMS, Western-Blot, and LEGENDplex™ analysis. Pattern of iris vasculature in JIAU patients was assessed qualitatively via fluorescein and indocyanine green angiography (FLA/ICGA). RESULTS: RNA-Seq of IT showed significantly differential expression (DE) of 136 genes between JIAU and POAG, of which 15 were associated with angiogenesis. qRT-PCR, performed to validate RNA-Seq results, showed upregulation of the angiogenesis-related genes Kdr, Angpt-1, Tie-1, Tie-2 and Mmrn2 in IT (JIAU vs POAG, p > 0.05). LC-IMS of IT revealed a total number of 56 DE proteins (JIAU vs POAG), of which Angiopoetin, Lumican and Decorin were associated with angiogenesis and showed increased (p > 0.05) expression on Western-Blot analysis. LEGENDplex™ analysis showed upregulation of ANGPT-2 in AH from JIAU compared to AAU and POAG, whereas VEGF was upregulated in AAU. Iris vascular leakage, hypoperfusion and neovascularization were observed by FLA/ICGA in JIA patients with treatment-refractory complicated course of uveitis. CONCLUSION: Angiogenesis-related factors could play a role in long-standing complicated JIAU, leading to clinically visible alterations in selected cases.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Artritis Juvenil / Uveítis / Trabeculectomía / Uveítis Anterior / Glaucoma de Ángulo Abierto Tipo de estudio: Risk_factors_studies Límite: Humans Idioma: En Revista: Mol Biol Rep Año: 2022 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Artritis Juvenil / Uveítis / Trabeculectomía / Uveítis Anterior / Glaucoma de Ángulo Abierto Tipo de estudio: Risk_factors_studies Límite: Humans Idioma: En Revista: Mol Biol Rep Año: 2022 Tipo del documento: Article País de afiliación: Alemania