Multiplexed site-specific genome engineering in <i>Mycolicibacterium neoaurum</i> by Att/Int system.

Liu, Ke; Lin, Gui-Hong; Liu, Kun; Liu, Yong-Jun; Tao, Xin-Yi; Gao, Bei; Zhao, Ming; Wei, Dong-Zhi; Wang, Feng-Qing

Multiplexed site-specific genome engineering in Mycolicibacterium neoaurum by Att/Int system.

Liu, Ke; Lin, Gui-Hong; Liu, Kun; Liu, Yong-Jun; Tao, Xin-Yi; Gao, Bei; Zhao, Ming; Wei, Dong-Zhi; Wang, Feng-Qing.

Afiliación

Liu K; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.
Lin GH; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.
Liu K; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.
Liu YJ; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.
Tao XY; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.
Gao B; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.
Zhao M; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.
Wei DZ; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.
Wang FQ; State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China.

Synth Syst Biotechnol ; 7(3): 1002-1011, 2022 Sep.

Article en En | MEDLINE | ID: mdl-35782483

Palabras clave

AE, allelic exchange; Att/Int, attachment/integration; BGCs, biosynthetic gene clusters; CRISPR, clustered regularly interspaced short palindromic repeats; DSBs, double-strand breaks; EGFP, enhanced green fluorescent proteins; EGT, ergothioneine; HPLC, high-performance liquid chromatography; HR, homologous recombination; MSGE, multiplexed site-specific genome engineering; Multi-copy integration; Mycolicibacterium; NHEJ, nonhomologous end-joining; PAM, protospacer adjacent motif; Phage integrase; Site-specific recombination; TB, tuberculosis; Xer recombinases; Xer-cise, Xer recombinases; aMSGE, advanced multiplex site-specific genome engineering; attB, bacterial attachment site; attP, phage attachment site; miSSR, multiplexed integrase-assisted site-specific recombination

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Synth Syst Biotechnol Año: 2022 Tipo del documento: Article País de afiliación: China

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