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The uncoating of EV71 in mature late endosomes requires CD-M6PR.
Ohka, Seii; Tan, Soon Hao; Ishiyama, Eri; Ogasawara, Katsutoshi; Hanasaka, Tomohito; Ishida, Kinji; Hagiwara, Kyoji; Liu, Chia-Chyi; Chong, Pele Choi-Sing; Hanaki, Ken-Ichi; Schiavo, Giampietro.
Afiliación
  • Ohka S; Neurovirology Project, Tokyo Metropolitan Institute of Medical Science, 156-8506, Tokyo, Japan.
  • Tan SH; Department of Pathology, University of Malaya, 59100, Kuala Lumpur, Malaysia.
  • Ishiyama E; Technical Support Center for Life Science Research, Iwate Medical University, 028-3694, Iwate, Japan.
  • Ogasawara K; Technical Support Center for Life Science Research, Iwate Medical University, 028-3694, Iwate, Japan.
  • Hanasaka T; Technical Support Center for Life Science Research, Iwate Medical University, 028-3694, Iwate, Japan.
  • Ishida K; Technical Support Center for Life Science Research, Iwate Medical University, 028-3694, Iwate, Japan.
  • Hagiwara K; Neurovirology Project, Tokyo Metropolitan Institute of Medical Science, 156-8506, Tokyo, Japan.
  • Liu CC; Vaccine R&D center, National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan Town, Miaoli County, 35053, Taiwan.
  • Chong PC; Vaccine R&D center, National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan Town, Miaoli County, 35053, Taiwan.
  • Hanaki KI; Technical Support Center for Life Science Research, Iwate Medical University, 028-3694, Iwate, Japan.
  • Schiavo G; Molecular NeuroPathobiology Laboratory, Queen Square Institute of Neurology, University College London, London, WC1N 3BG, United Kingdom.
Biol Open ; 11(9)2022 09 15.
Article en En | MEDLINE | ID: mdl-35929543
ABSTRACT
Enterovirus 71 (EV71) is one of the causative agents of hand-foot-and-mouth disease, which in some circumstances could lead to severe neurological diseases. Despite of its importance for human health, little is known about the early stages of EV71 infection. EV71 starts uncoating with its receptor, human scavenger receptor B2 (hSCARB2), at low pH. We show that EV71 was not targeted to lysosomes in human rhabdomyosarcoma cells overexpressing hSCARB2 and that the autophagic pathway is not essential for EV71 productive uncoating. Instead, EV71 was efficiently uncoated 30 min after infection in late endosomes (LEs) containing hSCARB2, mannose-6-phosphate receptor (M6PR), RAB9, bis(monoacylglycero)phosphate and lysosomal associated membrane protein 2 (LAMP2). Furthering the notion that mature LEs are crucial for EV71 uncoating, cation-dependent (CD)-M6PR knockdown impairs EV71 infection. Since hSCARB2 interacts with cation-independent (CI)-M6PR through M6P-binding sites and CD-M6PR also harbor a M6P-binding site, CD-M6PR is likely to play important roles in EV71 uncoating in LEs.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Enterovirus / Enterovirus Humano A / Infecciones por Enterovirus Límite: Animals / Humans Idioma: En Revista: Biol Open Año: 2022 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Enterovirus / Enterovirus Humano A / Infecciones por Enterovirus Límite: Animals / Humans Idioma: En Revista: Biol Open Año: 2022 Tipo del documento: Article País de afiliación: Japón