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Effective Rapid Diagnosis of Bacterial and Fungal Bloodstream Infections by T2 Magnetic Resonance Technology in the Pediatric Population.
Lucignano, Barbara; Cento, Valeria; Agosta, Marilena; Ambrogi, Federico; Albitar-Nehme, Sami; Mancinelli, Livia; Mattana, Giordana; Onori, Manuela; Galaverna, Federica; Di Chiara, Luca; Fragasso, Tiziana; Bianchi, Roberto; Tortora, Francesca; Auriti, Cinzia; Dotta, Andrea; Cecchetti, Corrado; Perdichizzi, Salvatore; Raponi, Massimiliano; Onetti Muda, Andrea; Nerini Molteni, Silvia; Villani, Alberto; Locatelli, Franco; Perno, Carlo Federico; Bernaschi, Paola.
Afiliación
  • Lucignano B; Unit of Microbiology and Diagnostic Immunology, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Cento V; Department of Biomedical Sciences, Humanitas University, Milan, Italy.
  • Agosta M; IRCCS Humanitas Research Hospital, Milan, Italy.
  • Ambrogi F; Unit of Microbiology and Diagnostic Immunology, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Albitar-Nehme S; Department of Clinical and Community Sciences, Università degli Studi di Milano, Milan, Italy.
  • Mancinelli L; Unit of Microbiology and Diagnostic Immunology, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Mattana G; Unit of Microbiology and Diagnostic Immunology, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Onori M; Unit of Microbiology and Diagnostic Immunology, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Galaverna F; Unit of Microbiology and Diagnostic Immunology, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Di Chiara L; Department of Pediatric Hematology and Oncology and of Cell and Gene Therapy, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Fragasso T; Department of Cardiology and Cardiac Surgery, Pediatric Cardiac Intensive Care Unit, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Bianchi R; Department of Cardiology and Cardiac Surgery, Pediatric Cardiac Intensive Care Unit, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Tortora F; Anesthesia and Critical Care Medicine, ARCO Rome, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Auriti C; Anesthesia and Critical Care Medicine, ARCO Rome, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Dotta A; Neonatal Intensive Care Unit, Medical and Surgical Department of the Fetus Newborn and Infant, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Cecchetti C; Neonatal Intensive Care Unit, Medical and Surgical Department of the Fetus Newborn and Infant, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Perdichizzi S; Pediatric Intensive Care Unit, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Raponi M; Pediatric Intensive Care Unit, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Onetti Muda A; Health Directorate, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Nerini Molteni S; Department of Laboratories, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Villani A; Chemical-Clinical and Microbiological Analysis, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
  • Locatelli F; General Pediatrics Unit, Pediatric Emergency and General Pediatrics Department, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Perno CF; Department of Pediatric Hematology and Oncology and of Cell and Gene Therapy, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
  • Bernaschi P; Sapienza University of Rome, Rome, Italy.
J Clin Microbiol ; 60(10): e0029222, 2022 Oct 19.
Article en En | MEDLINE | ID: mdl-36069557
ABSTRACT
Children are prone to bloodstream infections (BSIs), the rapid and accurate diagnosis of which is an unmet clinical need. The T2MR technology is a direct molecular assay for identification of BSI pathogens, which can help to overcome the limits of blood culture (BC) such as diagnostic accuracy, blood volumes required, and turnaround time. We analyzed results obtained with the T2Bacteria (648) and T2Candida (106) panels in pediatric patients of the Bambino Gesù Children's Hospital between May 2018 and September 2020 in order to evaluate the performance of the T2Dx instrument with respect to BC. T2Bacteria and T2Candida panels showed 84.2% and 100% sensitivity with 85.9% and 94.1% specificity, respectively. The sensitivity and specificity of the T2Bacteria panel increased to 94.9% and 98.7%, respectively, when BC was negative but other laboratory data supported the molecular result. T2Bacteria sensitivity was 100% with blood volumes <2 mL in neonates and infants. T2Bacteria and T2Candida provided definitive microorganism identification in a mean time of 4.4 and 3.7 h, respectively, versus 65.7 and 125.5 h for BCs (P < 0.001). T2 panels rapidly and accurately enable a diagnosis of a pediatric BSI, even in children under 1 year of age and for very small blood volumes. These findings support their clinical use in life-threatening pediatric infections, where the time to diagnosis is of utmost importance, in order to improve survival and minimize the long-term sequalae of sepsis. The T2 technology could be further developed to include more bacteria and fungi species that are involved in the etiology of sepsis.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Sepsis / Micosis Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Child / Humans / Newborn Idioma: En Revista: J Clin Microbiol Año: 2022 Tipo del documento: Article País de afiliación: Italia

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Sepsis / Micosis Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Child / Humans / Newborn Idioma: En Revista: J Clin Microbiol Año: 2022 Tipo del documento: Article País de afiliación: Italia