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Phytochemical Evaluation and Anti-Inflammatory Potential of Miconia albicans (Sw.) Triana Extracts.
Manzano, Mariana Inocencio; Centa, Ariana; Veiga, Alan de Almeida; da Costa, Nayara Souza; Bonatto, Sandro J R; de Souza, Lauro M; Smiderle, Fhernanda Ribeiro.
Afiliación
  • Manzano MI; Instituto de Pesquisa Pelé Pequeno Príncipe, Av. Silva Jardim, 1632, Água Verde, Curitiba CEP 80250-060, PR, Brazil.
  • Centa A; Faculdades Pequeno Príncipe, Av. Iguaçu, 333, Rebouças, Curitiba CEP 80230-020, PR, Brazil.
  • Veiga AA; Instituto de Pesquisa Pelé Pequeno Príncipe, Av. Silva Jardim, 1632, Água Verde, Curitiba CEP 80250-060, PR, Brazil.
  • da Costa NS; Laboratório de Pesquisa Translacional em Saúde, Universidade Alto Vale do Rio do Peixe, Rua Victor Baptista Adami, 800, Centro, Caçador CEP 89500-000, SC, Brazil.
  • Bonatto SJR; Instituto de Pesquisa Pelé Pequeno Príncipe, Av. Silva Jardim, 1632, Água Verde, Curitiba CEP 80250-060, PR, Brazil.
  • de Souza LM; Instituto de Pesquisa Pelé Pequeno Príncipe, Av. Silva Jardim, 1632, Água Verde, Curitiba CEP 80250-060, PR, Brazil.
  • Smiderle FR; Faculdades Pequeno Príncipe, Av. Iguaçu, 333, Rebouças, Curitiba CEP 80230-020, PR, Brazil.
Molecules ; 27(18)2022 Sep 13.
Article en En | MEDLINE | ID: mdl-36144693
ABSTRACT
The plant Miconia albicans (Sw.) Triana has been popularly used in Brazil to treat chronic inflammatory disturbances, such as osteoarthritis. This disease affects 250 million people worldwide, and is associated with intense pain and loss of articular function. There is a lack of information about the phytochemistry and bioactivity of M. albicans. Therefore, this study determined the chemical composition of some extracts and evaluated their cytotoxicity, along with their antioxidant and anti-inflammatory, activities using in vitro models. Aqueous and ethanolic extracts were prepared. Afterwards, a liquid-liquid partition was developed using chloroform, ethyl acetate, and n-butanol. The extracts were characterized by LC-MS, and their biological activities were evaluated on epithelial cells (Vero), tumoral hepatic cells (Hep-G2), and THP-1 macrophages. LC-MS analyses identified several flavonoids in all fractions, such as quercetin, myricetin, and their glycosides. The crude extracts and n-butanol fractions did not present cytotoxicity to the cells. The non-toxic fractions presented significant antioxidant activity when evaluated in terms of DPPH scavenging activity, lipid peroxidation, and ROS inhibition. THP-1 macrophages treated with the n-butanol fraction (250 µg/mL) released fewer pro-inflammatory cytokines, even in the presence of LPS. In the future, it will be necessary to identify the phytochemicals that are responsible for anti-inflammatory effects for the discovery of new drugs. In vivo studies on M. albicans extracts are still required to confirm their possible mechanisms of action.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Melastomataceae Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Brasil

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Melastomataceae Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Brasil