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Molecular characterization of aviadenovirus serotypes and pathogenicity of the identified adenovirus in broiler chickens.
Lebdah, Mohamed; Alshaya, Dalal S; Jalal, Areej S; Mousa, Mohamed R; Radwan, Mohamed M; Samir, Mahmoud; Adel, Amany; Albaqami, Najah M; El-Saadony, Mohamed T; El-Tarabily, Khaled A; El Basrey, Yara F H.
Afiliación
  • Lebdah M; Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt.
  • Alshaya DS; Biology Department, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, 11671, Saudi Arabia.
  • Jalal AS; Biology Department, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, 11671, Saudi Arabia.
  • Mousa MR; Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, 11361, Egypt.
  • Radwan MM; Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza, 11361, Egypt.
  • Samir M; Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, Giza, 12618, Egypt.
  • Adel A; Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, Giza, 12618, Egypt.
  • Albaqami NM; Department of Biological Sciences, Zoology, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
  • El-Saadony MT; Department of Agricultural Microbiology, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt.
  • El-Tarabily KA; Department of Biology, College of Science, United Arab Emirates University, Al-Ain, 15551, United Arab Emirates; Khalifa Center for Genetic Engineering and Biotechnology, United Arab Emirates University, Al-Ain, 15551, United Arab Emirates; Harry Butler Institute, Murdoch University, Murdoch, Wester
  • El Basrey YFH; Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt.
Poult Sci ; 101(12): 101918, 2022 Dec.
Article en En | MEDLINE | ID: mdl-36209666
Inclusion body hepatitis (IBH) is an economically significant viral disease that primarily affects broiler chickens. At least 12 different aviadenovirus serotypes are responsible for causing IBH. This study aimed to use polymerase chain reaction (PCR) and phylogenetic analysis to characterize fowl adenovirus isolates that were in circulation from 2019 to 2021 and investigate the pathogenicity of the isolated strains in commercial broiler chickens. Suspected liver samples were molecularly identified using hexon gene targeting by PCR, and viruses were isolated using chick embryo liver cell culture. For serotype identification, the fowl adenovirus-positive samples were subjected to hexon gene sequencing and phylogenetic analysis. The pathogenicity of two isolates was tested in commercial chickens via the oral route. The phylogenetic analysis of the hexon gene showed that the isolated viruses clustered with serotype 8a species E. On testing the pathogenicity of the isolates based on necropsy and histopathological examination, no mortality was observed; however, lesions were observed in the liver, kidney, heart, pancreas, bursa, and lung specimens with intermittent virus shedding at different time points throughout the experimental period. Further research on the likelihood of vaccine production is warranted to limit disease-related losses.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Enfermedades de las Aves de Corral / Infecciones por Adenoviridae / Aviadenovirus Límite: Animals Idioma: En Revista: Poult Sci Año: 2022 Tipo del documento: Article País de afiliación: Egipto

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Enfermedades de las Aves de Corral / Infecciones por Adenoviridae / Aviadenovirus Límite: Animals Idioma: En Revista: Poult Sci Año: 2022 Tipo del documento: Article País de afiliación: Egipto