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Neoplastic cell enrichment of tumor tissues using coring and laser microdissection for proteomic and genomic analyses of pancreatic ductal adenocarcinoma.
Li, Qing Kay; Hu, Yingwei; Chen, Lijun; Schnaubelt, Michael; Cui Zhou, Daniel; Li, Yize; Lu, Rita Jui-Hsien; Thiagarajan, Mathangi; Hostetter, Galen; Newton, Chelsea J; Jewell, Scott D; Omenn, Gil; Robles, Ana I; Mesri, Mehdi; Bathe, Oliver F; Zhang, Bing; Ding, Li; Hruban, Ralph H; Chan, Daniel W; Zhang, Hui.
Afiliación
  • Li QK; Department of Pathology, the Johns Hopkins University, 400 N Broadway, Smith Bldg Rm 4011, Baltimore, MD, 21231, USA. qli23@jhmi.edu.
  • Hu Y; Department of Oncology, Sidney Kimmel Cancer Center, Johns Hopkins Medical Institutions, 600 N. Wolfe Street, Baltimore, MD, USA. qli23@jhmi.edu.
  • Chen L; Department of Pathology, the Johns Hopkins University, 400 N Broadway, Smith Bldg Rm 4011, Baltimore, MD, 21231, USA.
  • Schnaubelt M; Department of Pathology, the Johns Hopkins University, 400 N Broadway, Smith Bldg Rm 4011, Baltimore, MD, 21231, USA.
  • Cui Zhou D; Department of Pathology, the Johns Hopkins University, 400 N Broadway, Smith Bldg Rm 4011, Baltimore, MD, 21231, USA.
  • Li Y; Department of Oncology, Washington University at Saint Louis, St Louis, MO, USA.
  • Lu RJ; Department of Oncology, Washington University at Saint Louis, St Louis, MO, USA.
  • Thiagarajan M; Department of Oncology, Washington University at Saint Louis, St Louis, MO, USA.
  • Hostetter G; Leidos Biomedical Research Inc, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
  • Newton CJ; Van Andel Research Institute, Grand Rapids, MI, USA.
  • Jewell SD; Van Andel Research Institute, Grand Rapids, MI, USA.
  • Omenn G; Van Andel Research Institute, Grand Rapids, MI, USA.
  • Robles AI; Department of Computational Medicine & Bioinformatics, University of Michigan, Ann Arbor, MI, USA.
  • Mesri M; Office of Cancer Clinical Proteomics Research, National Cancer Institute, Rockville, MD, USA.
  • Bathe OF; Office of Cancer Clinical Proteomics Research, National Cancer Institute, Rockville, MD, USA.
  • Zhang B; Department of Surgery and Oncology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
  • Ding L; Lester and Sue Smith Breast Center, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
  • Hruban RH; Department of Oncology, Washington University at Saint Louis, St Louis, MO, USA.
  • Chan DW; Department of Pathology, the Johns Hopkins University, 400 N Broadway, Smith Bldg Rm 4011, Baltimore, MD, 21231, USA.
  • Zhang H; Department of Oncology, Sidney Kimmel Cancer Center, Johns Hopkins Medical Institutions, 600 N. Wolfe Street, Baltimore, MD, USA.
Clin Proteomics ; 19(1): 36, 2022 Oct 20.
Article en En | MEDLINE | ID: mdl-36266629
BACKGROUND: The identification of differentially expressed tumor-associated proteins and genomic alterations driving neoplasia is critical in the development of clinical assays to detect cancers and forms the foundation for understanding cancer biology. One of the challenges in the analysis of pancreatic ductal adenocarcinoma (PDAC) is the low neoplastic cellularity and heterogeneous composition of bulk tumors. To enrich neoplastic cells from bulk tumor tissue, coring, and laser microdissection (LMD) sampling techniques have been employed. In this study, we assessed the protein and KRAS mutation changes associated with samples obtained by these enrichment techniques and evaluated the fraction of neoplastic cells in PDAC for proteomic and genomic analyses. METHODS: Three fresh frozen PDAC tumors and their tumor-matched normal adjacent tissues (NATs) were obtained from three sampling techniques using bulk, coring, and LMD; and analyzed by TMT-based quantitative proteomics. The protein profiles and characterizations of differentially expressed proteins in three sampling groups were determined. These three PDACs and samples of five additional PDACs obtained by the same three sampling techniques were also subjected to genomic analysis to characterize KRAS mutations. RESULTS: The neoplastic cellularity of eight PDACs ranged from less than 10% to over 80% based on morphological review. Distinctive proteomic patterns and abundances of certain tumor-associated proteins were revealed when comparing the tumors and NATs by different sampling techniques. Coring and bulk tissues had comparable proteome profiles, while LMD samples had the most distinct proteome composition compared to bulk tissues. Further genomic analysis of bulk, cored, or LMD samples demonstrated that KRAS mutations were significantly enriched in LMD samples while coring was less effective in enriching for KRAS mutations when bulk tissues contained a relatively low neoplastic cellularity. CONCLUSIONS: In addition to bulk tissues, samples from LMD and coring techniques can be used for proteogenomic studies. The greatest enrichment of neoplastic cellularity is obtained with the LMD technique.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Clin Proteomics Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Clin Proteomics Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos