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In Situ Hybridization as a Method to Examine Gene Regulatory Activity In Vivo.
Datta, Rhea R; Onal, Pinar.
Afiliación
  • Datta RR; Department of Biology, Hamilton College, Clinton, NY, USA. rdatta@hamilton.edu.
  • Onal P; Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA. pinar.onal@bilkent.edu.tr.
Methods Mol Biol ; 2599: 241-254, 2023.
Article en En | MEDLINE | ID: mdl-36427154
Transcription factor-enhancer binding events are among the most well-studied protein-DNA interactions, allowing researchers to determine mechanisms of transcriptional activation or repression during development. While large-scale ChIP-sequence datasets, together with computational predictions and chromatin accessibility data, yield information on potential transcription factor binding activities, reporter gene assays provide measurable information on whether these binding activities are functional in particular cell types during development. Here, we present a detailed protocol to examine enhancer activity in Drosophila embryos using cloning, transgenesis, and in situ hybridization.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Investigadores / Regulación de la Expresión Génica Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Investigadores / Regulación de la Expresión Génica Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos