3-Hydroxyflavone - bovine serum albumin complex used as fluorescent probe for the detection of pepsin.
Anal Biochem
; 662: 115003, 2023 02 01.
Article
en En
| MEDLINE
| ID: mdl-36476742
ABSTRACT
In this study, a method for the determination of pepsin using a 3-hydroxyflavone (3-HF)-bovine serum albumin (BSA) complex as a fluorescent probe was established. Under acidic conditions, 3-HF can react with BSA to emit strong green fluorescence, which involves an excited state intramolecular proton transfer and fluorescence resonance energy transfer. After pepsin is added, the fluorescence of the 3-HF-BSA system is quenched due to the hydrolysis of BSA. The degree of 3-HF-BSA fluorescence quenching has a good linear relationship with the concentration of pepsin in the range of 10-80 µg/mL. The correlation coefficient was R2 = 0.9932 and the detection limit was 2.41 µg/mL. This method has high selectivity and good stability and has been used to determine pepsin in real samples with satisfactory results.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Albúmina Sérica Bovina
/
Colorantes Fluorescentes
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Anal Biochem
Año:
2023
Tipo del documento:
Article
País de afiliación:
China