Conversion of waste cooked rice into maltose by a Bacillus subtilis-derived maltogenic amylase and production of maltobionic acid from the prepared maltose using genetically modified Pseudomonas taetrolens.
Bioprocess Biosyst Eng
; 46(2): 273-277, 2023 Feb.
Article
en En
| MEDLINE
| ID: mdl-36526877
ABSTRACT
In this study, we attempted to produce maltobionic acid (MBA) from waste cooked rice (WCR) using maltose as an intermediate. In our previous study, we produced maltose from WCR using a commercial maltogenic amylase (Maltogenase L). However, in the present study, we used wild-type Bacillus subtilis, which inherently produces maltogenic amylase (AmyE), instead of Maltogenase L to produce maltose from WCR. During cultivation of B. subtilis with WCR, maltose was successfully produced by AmyE in the culture medium. To improve maltose production, we constructed a recombinant B. subtilis strain expressing AmyE and used it for maltose production. Following cultivation of the recombinant B. subtilis strain, the maltose production titer (34.6 g/L) increased approximately 3.6-fold that (9.6 g/L) obtained from the cultivation of wild-type B. subtilis. Using Pseudomonas taetrolens, an efficient MBA-producing bacterium, 28.8 g/L of MBA was produced from the prepared maltose (27.6 g/L). The above results indicated that MBA was successfully produced from WCR via a two-step process, which involved the conversion of WCR into maltose by maltogenic amylase-producing B. subtilis and the production of MBA from the WCR-derived maltose by P. taetrolens.
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Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oryza
/
Bacillus subtilis
Idioma:
En
Revista:
Bioprocess Biosyst Eng
Asunto de la revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
Año:
2023
Tipo del documento:
Article