Cloning and characterization of thermostable amylopullulanase TbbApu and its C-terminal truncated variants with enhanced activity in organic solvents.

Mumcu, Hande; Kayrav, Aycan; Isleyen, Naciye Durmus; Karaguler, Nevin Gul

Mumcu, Hande; Kayrav, Aycan; Isleyen, Naciye Durmus; Karaguler, Nevin Gul.

Afiliación

Mumcu H; Istanbul Technical University, Faculty of Science and Letters, Department of Molecular Biology and Genetics, 34469 Istanbul, Turkey; Istanbul Technical University, Dr. Orhan Ocalgiray Molecular Biology-Biotechnology and Genetics Research Center, Istanbul, Turkey.
Kayrav A; Istanbul Technical University, Faculty of Science and Letters, Department of Molecular Biology and Genetics, 34469 Istanbul, Turkey; Istanbul Technical University, Dr. Orhan Ocalgiray Molecular Biology-Biotechnology and Genetics Research Center, Istanbul, Turkey.
Isleyen ND; Istanbul Technical University, Faculty of Science and Letters, Department of Molecular Biology and Genetics, 34469 Istanbul, Turkey; Istanbul Technical University, Dr. Orhan Ocalgiray Molecular Biology-Biotechnology and Genetics Research Center, Istanbul, Turkey.
Karaguler NG; Istanbul Technical University, Faculty of Science and Letters, Department of Molecular Biology and Genetics, 34469 Istanbul, Turkey; Istanbul Technical University, Dr. Orhan Ocalgiray Molecular Biology-Biotechnology and Genetics Research Center, Istanbul, Turkey. Electronic address: karaguler@itu.ed

Enzyme Microb Technol ; 164: 110176, 2023 Mar.

Article en En | MEDLINE | ID: mdl-36529061

Asunto(s)

Proteínas Bacterianas; Glicósido Hidrolasas; Thermoanaerobacter; Proteínas Bacterianas/metabolismo; Clonación Molecular; Estabilidad de Enzimas; Escherichia coli/genética; Escherichia coli/metabolismo; Glicósido Hidrolasas/química; Glicósido Hidrolasas/genética; Concentración de Iones de Hidrógeno; Solventes/química; Almidón/metabolismo; Especificidad por Sustrato; Thermoanaerobacter/enzimología

Palabras clave

C-terminal truncation; Organic solvent enhancement; Thermostable amylopullulanase

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Thermoanaerobacter / Glicósido Hidrolasas Idioma: En Revista: Enzyme Microb Technol Año: 2023 Tipo del documento: Article País de afiliación: Turquía

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