In situ cryo-FIB/SEM Specimen Preparation Using the Waffle Method.

Klykov, Oleg; Bobe, Daija; Paraan, Mohammadreza; Johnston, Jake D; Potter, Clinton S; Carragher, Bridget; Kopylov, Mykhailo; Noble, Alex J

Klykov, Oleg; Bobe, Daija; Paraan, Mohammadreza; Johnston, Jake D; Potter, Clinton S; Carragher, Bridget; Kopylov, Mykhailo; Noble, Alex J.

Afiliación

Klykov O; National Center for In-situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
Bobe D; National Center for In-situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
Paraan M; National Center for In-situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
Johnston JD; National Center for In-situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
Potter CS; Department of Physiology and Cellular Biophysics, Columbia University, New York, NY, USA.
Carragher B; National Center for In-situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
Kopylov M; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA; Contributed equally to this work.
Noble AJ; National Center for In-situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.

Bio Protoc ; 12(21)2022 Nov 05.

Article en En | MEDLINE | ID: mdl-36618877

Palabras clave

AutoTEM Cryo; FIB-SEM; HPF; In situ; Sample preparation; Waffle Method; cryo-ET

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Bio Protoc Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos

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