Integrating high-throughput exposure assessment and in vitro screening data to prioritize endocrine-active potential and dietary risks of pesticides and veterinary drug residues in animal products.
Food Chem Toxicol
; 173: 113639, 2023 Mar.
Article
en En
| MEDLINE
| ID: mdl-36708863
ABSTRACT
New approach methodologies in toxicology, such as in vitro high-throughput screening (HTS), can minimize the use of experimental animals and allow mechanism-based predictions of in vivo toxicity. HTS data has been increasingly used in the regulatory context; however, only a few studies integrated dietary exposure and HTS data to foster chemical prioritization in food. Additionally, the endocrine-associated risk of veterinary drug residues in food is yet to be fully characterized. This study aims to systematically compare the translated HTS data with the acceptable daily intake (ADI) values and prioritize the pesticides and veterinary drug residues (n = 294) in food using the exposure-activity ratio (EAR) and Toxicological Prioritization index (ToxPi). The dietary exposure assessment was accomplished using a stochastic human exposure and dose simulation high-throughput model (SHEDS-HT). We selected 76 HTS assays from 12 nuclear receptors to represent the molecular initiating event (MIE) of endocrine-disrupting phenotypes. Chemical prioritization was achieved using 4 methods (i.e., EAR-OED, EAR-ADI, ToxPi-exposure + ADI, and ToxPi-exposure + endocrine score), where the consensus prioritized chemicals were fipronil, furazolidone, oxolinic acid, and oxytetracycline for the Taiwanese population. This case study demonstrates the utility of HTS data in fostering regulatory decisions on chemicals, especially for those lacking comprehensive toxicity data.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Plaguicidas
/
Drogas Veterinarias
Tipo de estudio:
Diagnostic_studies
/
Etiology_studies
/
Prognostic_studies
/
Risk_factors_studies
/
Screening_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Food Chem Toxicol
Año:
2023
Tipo del documento:
Article