Systematic analysis of the oncogenic role of FAM83D across cancers based on data mining.

ABSTRACT

Family with sequence similarity of 83D (FAM83D) is overexpressed in various cancers. However, no pan-cancer analysis is presently available. In the present study, we used a bioinformatics analysis to explore the diagnostic and prognostic value of FAM83D expression levels in human cancers. The GEPIA 2, TIMER 2.0, ENCORI, and DriverDBV3 databases were used to evaluate FAM83D expression levels. The potential prognostic value of FAM83D expression was analyzed using the GEPIA 2, UALCAN, and TISIB databases. The driver gene and promoter methylation levels regarding FAM83D were evaluated using the TIMER 2.0 and UALCAN databases. To further analyze interactive networks for FAM83D, FAM83D-binding proteins and related genes were determined using STRING and Gene MANIA analytic tools. Highly expressed FAM83D could be associated with mutated TP53 and promoter DNA methylation. Relative network analysis suggested that FAM83D was mainly involved in the progesterone-mediated oocyte maturation pathway, cell cycle regulation, and several other signaling pathways. Therefore, the differential expression of FAM83D could serve as a diagnostic and prognostic biomarker for various cancers. Our study revealed useful information about the differential expression of FAM83D, prognostic values, and potential functional networks in a variety of cancers, providing valuable substantive and methodological information to explore the underlying mechanisms.Abbreviations BP Biological processes; CC Cellular components; DAVID Database for Annotation, Visualization, and Integrated Discovery; DFS Disease-free survival; ENCORI Encyclopedia of RNA Interactomes; FAM83 Family with sequence similarity 83; FAM83D Family with sequence similarity of 83D; GEO Gene Expression Omnibus; GEPIAx2 Gene Expression Profiling Interactive Analysis 2; GO Gene Ontology; GTEx Genotype-Tissue Expression; KEGG Kyoto Encyclopedia of Genes and Genomes; KIRC Kidney renal clear cell carcinoma; LIHC Liver hepatocellular carcinoma; LUAD Lung adenocarcinoma; MF Molecular functions miRNA microRNA; OS Overall survival; PAAD Pancreatic adenocarcinoma; PPI Protein - protein interaction; RNA-seq RNA-sequencing; TCGA The Cancer Genome Atlas; TIMER 2.0 Tumor Immune Estimation Resource 2.0; UALCAN University of Alabama at Birmingham Cancer; UCEC Uterine corpus endometrial carcinoma.