Immunostaining of intact C. elegans using polyacrylamide embedding.
STAR Protoc
; 4(1): 101956, 2023 03 17.
Article
en En
| MEDLINE
| ID: mdl-36856764
ABSTRACT
A major barrier to immunostaining Caenorhabditis elegans is the permeabilization of the worm's cuticle without distorting or damaging its body. We present here a gel-based immobilization protocol for fixed worms coupled with chemical and enzymatic permeabilization. The permeabilization is followed by antibody staining and fluorescent imaging. This protocol can be modified for different fixatives, permeabilizing reagents, or molecular readouts. Unlike previous immunostaining approaches, such as freeze cracking or dissection, this protocol enables immunostaining across the whole body of a well-preserved C. elegans.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Resinas Acrílicas
/
Caenorhabditis elegans
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2023
Tipo del documento:
Article