The effect of Azo-dyes on glioblastoma cells in vitro.
Saudi J Biol Sci
; 30(3): 103599, 2023 Mar.
Article
en En
| MEDLINE
| ID: mdl-36874201
ABSTRACT
Despite the multidisciplinary standard treatment of glioblastoma (GB) consisting of maximal surgical resection, followed by radiotherapy (RT) plus concomitant chemotherapy with temozolomide (TMZ), the majority of patients experience tumor progression and almost universal mortality. In recent years, efforts have been made to create new agents for GB treatment, of which azo-dyes proved to be potential candidates, showing antiproliferative effects by inducing apoptosis and by inhibiting different signaling pathways. In this study we evaluated the antiproliferative the effect of six azo-dyes and TMZ on a low passage human GB cell line using MTT assay. We found that all compounds proved antiproliferative properties on GB cells. At equimolar concentrations azo-dyes induced more cytotoxic effect than TMZ. We found that Methyl Orange required the lowest IC50 for 3 days of treatment (26.4684 µM), whilst for 7 days of treatment, two azo dyes proved to have the highest potency Methyl Orange IC50 = 13.8808 µM and Sudan I IC50 = 12.4829 µM. The highest IC50 was determined for TMZ under both experimental situations. Conclusions:
Our research represents a novelty, by offering unique valuable data regarding the azo-dye cyototoxic effects in high grade brain tumors. This study may focus the attention on azo-dye agents that may represent an insufficient exploited source of agents for cancer treatment.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Idioma:
En
Revista:
Saudi J Biol Sci
Año:
2023
Tipo del documento:
Article
País de afiliación:
Rumanía