Chemical and in vitro toxicological comparison of emissions from a heated tobacco product and the 1R6F reference cigarette.
Toxicol Rep
; 10: 281-292, 2023.
Article
en En
| MEDLINE
| ID: mdl-36876026
ABSTRACT
It has previously been found that, compared with cigarette smoke, the aerosols generated by heated tobacco products contain fewer and lower harmful and potentially harmful constituents (HPHCs) and elicit lower biological activity in in vitro models and lower smoking-related exposure biomarker levels in clinical studies. It is important to accumulate such scientific evidences for heated tobacco products with a novel heating system, because different heating system may affect the quantitative aspect of the amount of HPHCs and the qualitative aspect of the biological activity of the aerosol generated. Here, the chemical properties of, and toxicological responses to aerosols emitted by DT3.0a, a new heated tobacco product with a novel heating system, and cigarette smoke (CS) were compared, using chemical analyses, in vitro battery (standardized genotoxicity and cytotoxicity) assays, and mechanistic (ToxTracker and two-dimensional cell culture) assays. Regular- and menthol-flavored DT3.0a and standard 1R6F reference cigarettes were tested. Selected HPHC yields were lower in DT3.0a aerosol than 1R6F CS. The genotoxicity-related assays indicated that DT3.0a aerosol was not genotoxic, regardless of metabolic activation. The other biological assays indicated that less cytotoxicity induction and oxidative stress response were elicited by DT3.0a aerosol compared with 1R6F CS. Similar results were found for both regular and menthol DT3.0a. Like previous reports for heated tobacco products with other heating systems, the results of this study indicated that DT3.0a aerosols have chemical and biological properties less likely to be harmful than 1R6F CS.
ACM, aerosol collected mass; ARE, anti-oxidant responsive element; AqE, aqueous extract; BDL, below detection limit; CMF-PBS, calcium- and magnesium-free phosphate buffered saline; CS, cigarette smoke; Cytotoxicity; DT3.0a, Direct Heating Tobacco System Platform 3 generation 3 version a; GFP, green fluorescent protein; GVP, gas-vapor phase; Genotoxicity; HPHCs; HPHCs, harmful and potentially harmful constituents; HTP, heated tobacco product; Heated tobacco product; IC50, half maximal inhibitory concentration; ISO, International Organization for Standardization; In vitro; LOD, limit of detection; LOQ, limit of quantification; MLA, mouse lymphoma assay; MN, micronucleus; MRTP, modified risk tobacco product; NQ, not quantified; NRU, neutral red uptake; Oxidative stress; ROS, reactive oxygen species; THP, tobacco heating product; THS, tobacco heating system; TPM, total particulate matter; mES, mouse embryonic stem
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Tipo de estudio:
Qualitative_research
Idioma:
En
Revista:
Toxicol Rep
Año:
2023
Tipo del documento:
Article
País de afiliación:
Japón