Contemporary Considerations for Establishing Reference Methods for Antibacterial Susceptibility Testing.
J Clin Microbiol
; 61(6): e0188622, 2023 06 20.
Article
en En
| MEDLINE
| ID: mdl-36971571
ABSTRACT
Antibacterial susceptibility testing (AST) is performed to guide therapy, perform resistance surveillance studies, and support development of new antibacterial agents. For 5 decades, broth microdilution (BMD) has served as the reference method to assess in vitro activity of antibacterial agents against which both novel agents and diagnostic tests have been measured. BMD relies on in vitro inhibition or killing of bacteria. It is associated with several limitations:
it is a poor mimic of the in vivo milieu of bacterial infections, requires multiple days to perform, and is associated with subtle, difficult to control variability. In addition, new reference methods will soon be needed for novel agents whose activity cannot be evaluated by BMD (e.g., those that target virulence). Any new reference methods must be standardized, correlated with clinical efficacy and be recognized internationally by researchers, industry, and regulators. Herein, we describe current reference methods for in vitro assessment of antibacterial activity and highlight key considerations for the generation of novel reference methods.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Antibacterianos
Límite:
Humans
Idioma:
En
Revista:
J Clin Microbiol
Año:
2023
Tipo del documento:
Article
País de afiliación:
Estados Unidos