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Generation and characterization of infectious molecular clones of transmitted/founder HIV-1 subtype C viruses.
Luthuli, Bonisile; Gounder, Kamini; Deymier, Martin J; Dong, Krista L; Balazs, Alejandro B; Mann, Jaclyn K; Ndung'u, Thumbi.
Afiliación
  • Luthuli B; Africa Health Research Institute, Durban, South Africa.
  • Gounder K; Africa Health Research Institute, Durban, South Africa; HIV Pathogenesis Programme, The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa.
  • Deymier MJ; The Ragon Institute of MGH, MIT and Harvard University, Cambridge, MA, USA.
  • Dong KL; The Ragon Institute of MGH, MIT and Harvard University, Cambridge, MA, USA.
  • Balazs AB; The Ragon Institute of MGH, MIT and Harvard University, Cambridge, MA, USA.
  • Mann JK; HIV Pathogenesis Programme, The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa.
  • Ndung'u T; Africa Health Research Institute, Durban, South Africa; HIV Pathogenesis Programme, The Doris Duke Medical Research Institute, University of KwaZulu-Natal, Durban, South Africa; The Ragon Institute of MGH, MIT and Harvard University, Cambridge, MA, USA; Division of Infection and Immunity, University
Virology ; 583: 14-26, 2023 06.
Article en En | MEDLINE | ID: mdl-37084644
ABSTRACT
The genetic diversity of HIV impedes vaccine development. Identifying the viral properties of transmitted/founder (T/F) variants may provide a common vaccine target. To study the biological nature of T/F viruses, we constructed full-length clones from women detected during Fiebig stage I acute HIV-1 infection (AHI) from heterosexual male-to-female (MTF) transmission; and clones after one year of infection using In-Fusion-based cloning. Eighteen full-length T/F clones were generated from 9 women and six chronic infection clones were from 2 individuals. All clones but one were non-recombinant subtype C. Three of the 5 T/F clones and 3 chronic clones tested replicated efficiently in PBMCs and utilised CCR5 coreceptor for cell entry. Transmitted/founder and chronic infection clones displayed heterogenous in vitro replicative capacity and resistance to type I interferon. T/F viruses had shorter Env glycoproteins and fewer N-linked glycosylation sites in Env. Our findings suggest MTF transmission may select viruses with compact envelopes.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Infecciones por VIH / VIH-1 Tipo de estudio: Prognostic_studies Límite: Female / Humans / Male Idioma: En Revista: Virology Año: 2023 Tipo del documento: Article País de afiliación: Sudáfrica
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Infecciones por VIH / VIH-1 Tipo de estudio: Prognostic_studies Límite: Female / Humans / Male Idioma: En Revista: Virology Año: 2023 Tipo del documento: Article País de afiliación: Sudáfrica