Transfection of Barley Leaf Protoplasts with a Fluorescently Tagged Fungal Effector for In Planta Localization Studies in Barley.
Methods Mol Biol
; 2659: 83-93, 2023.
Article
en En
| MEDLINE
| ID: mdl-37249887
ABSTRACT
The isolation and transfection of protoplasts from plant leaves have been routinely used for transient expression and functional studies in model plants. However, current approaches to characterize pathogen effector molecules in a cereal host are inefficient and technically challenging. In this chapter, we describe a protocol to isolate and transfect barley mesophyll protoplasts with a fluorescently tagged fungal effector of the barley smut pathogen Ustilago hordei. Tagging of a fungal effector with a fluorescent protein and tracking its localization in cells of its natural host provides insight into its putative in planta localization and helps to narrow down the location of putative host interactors.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Hordeum
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2023
Tipo del documento:
Article
País de afiliación:
Canadá