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Transcriptome analysis reveals the molecular mechanisms by which carbon dots regulate the growth of Chlamydomonas reinhardtii.
Xue, Huidan; Dong, Yibei; Li, Zhihuan; Wang, Jing; Yuan, Xiaolong; He, Fei; Li, Zhengke; Gao, Xiang; Liu, Jianxi.
Afiliación
  • Xue H; School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China; School of Ecology and Environment, Northwestern Polytechnical University, Xi'an 710012, China. Electronic address: huidanxue@sust.edu.cn.
  • Dong Y; School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
  • Li Z; State Key Laboratory of Solidification Processing, Center of Advanced Lubrication and Seal Materials, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi'an 710072, China.
  • Wang J; School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
  • Yuan X; School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
  • He F; School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
  • Li Z; School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
  • Gao X; School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
  • Liu J; State Key Laboratory of Solidification Processing, Center of Advanced Lubrication and Seal Materials, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi'an 710072, China. Electronic address: jianxiliu@nwpu.edu.cn.
J Colloid Interface Sci ; 649: 22-35, 2023 Nov.
Article en En | MEDLINE | ID: mdl-37331107
ABSTRACT
Carbon dots (CDs) have attracted increasing attention for their ability to artificially improve photosynthesis. Microalgal bioproducts have emerged as promising sources of sustainable nutrition and energy. However, the gene regulation mechanism of CDs on microalgae remains unexplored. The study synthesized red-emitting CDs and applied them to Chlamydomonas reinhardtii. Results showed that 0.5 mg/L-CDs acted as light supplements to promote cell division and biomass in C. reinhardtii. CDs improved the energy transfer of PS II, photochemical efficiency of PS II, and photosynthetic electron transfer. The pigment content and carbohydrate production slightly increased, while protein and lipid contents significantly increased (by 28.4% and 27.7%, respectively) in a short cultivation time. Transcriptome analysis identified 1166 differentially expressed genes. CDs resulted in faster cell growth by up-regulating the expression of genes associated with cell growth and death, promoting sister chromatid separation, accelerating the mitotic process and shortening the cell cycle. CDs improved the ability of energy conversion by up-regulating photosynthetic electron transfer-related genes. Carbohydrate metabolism-related genes were regulated and provided more available pyruvate for the citrate cycle. The study provides evidence for the genetic regulation of microalgal bioresources by artificially synthesized CDs.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Chlamydomonas reinhardtii Idioma: En Revista: J Colloid Interface Sci Año: 2023 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Chlamydomonas reinhardtii Idioma: En Revista: J Colloid Interface Sci Año: 2023 Tipo del documento: Article