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Identification of universal stress proteins in wheat and functional characterization during abiotic stress.
Singh, Arunima; Singhal, Chanchal; Sharma, Arun Kumar; Khurana, Paramjit.
Afiliación
  • Singh A; Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India.
  • Singhal C; Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India.
  • Sharma AK; Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India.
  • Khurana P; Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India. param@genomeindia.org.
Plant Cell Rep ; 42(9): 1487-1501, 2023 Sep.
Article en En | MEDLINE | ID: mdl-37341826
ABSTRACT
KEY MESSAGE TaUSPs are localized in Endoplasmic reticulum and form homo and hetero dimers within themselves. They play significant role in multiple abiotic stress responses in yeast heterologous system and in plants. Universal Stress Proteins are stress responsive proteins present in a variety of life forms ranging from bacteria to multicellular plants and animals. In this study we have identified 85 TaUSP genes in the wheat genome and have characterised their abiotic stress responsive members in yeast under different stress conditions. Localization and Y2H studies suggest that wheat, USP proteins are localized in the ER complex, and extensively crosstalk amongst themselves through forming hetero and homodimers. Expression analysis of these TaUSP genes suggests their role in adaptation to multiple abiotic stresses. TaUSP_5D-1 was found to have some DNA binding activity in yeast. Certain abiotic stress responsive TaUSP genes are found to impart tolerance to temperature stress, oxidative stress, ER stress (DTT treatment) and LiCl2 stress in the yeast heterologous system. TaUSP_5D-1 overexpression in A. thaliana imparts drought tolerance via better lateral root network in transgenic lines. The TaUSP represents an important repertoire of genes for engineering abiotic stress responsiveness in crop plants.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Plantas / Triticum Tipo de estudio: Diagnostic_studies Idioma: En Revista: Plant Cell Rep Asunto de la revista: BOTANICA Año: 2023 Tipo del documento: Article País de afiliación: India

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Plantas / Triticum Tipo de estudio: Diagnostic_studies Idioma: En Revista: Plant Cell Rep Asunto de la revista: BOTANICA Año: 2023 Tipo del documento: Article País de afiliación: India