Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression.
Cell Struct Funct
; 48(2): 135-144, 2023 Jul 29.
Article
en En
| MEDLINE
| ID: mdl-37394513
ABSTRACT
We cloned and characterized two new coral fluorescent proteins h2-3 and 1-41. h2-3 formed an obligate dimeric complex and exhibited bright green fluorescence. On the other hand, 1-41 formed a highly multimeric complex and exhibited dim red fluorescence. We engineered 1-41 into AzaleaB5, a practically useful red-emitting fluorescent protein for cellular labeling applications. We fused h2-3 and AzaleaB5 to the ubiquitination domains of human Geminin and Cdt1, respectively, to generate a new color variant of Fucci (Fluorescent Ubiquitination-based Cell-Cycle Indicator) Fucci5. We found Fucci5 provided more reliable nuclear labeling for monitoring cell-cycle progression than the 1st and 2nd generations that used mAG/mKO2 and mVenus/mCherry, respectively.Key words fluorescent protein, cell cycle, time-lapse imaging, flow cytometry.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas de Ciclo Celular
/
Colorantes
Límite:
Humans
Idioma:
En
Revista:
Cell Struct Funct
Año:
2023
Tipo del documento:
Article