Optical coherence tomography-based design for a real-time motion corrected scanning microscope.
Opt Lett
; 48(14): 3805-3808, 2023 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-37450755
ABSTRACT
While two-photon fluorescence microscopy is a powerful platform for the study of functional dynamics in living cells and tissues, the bulk motion inherent to these applications causes distortions. We have designed a motion tracking module based on spectral domain optical coherence tomography which compliments a laser scanning two-photon microscope with real-time corrective feedback. The module can be added to fluorescent imaging microscopes using a single dichroic and without additional contrast agents. We demonstrate that the system can track lateral displacements as large as 10 µm at 5 Hz with latency under 14 ms and propose a scheme to extend the system to 3D correction with the addition of a remote focusing module. We also propose several ways to improve the module's performance by reducing the feedback latency. We anticipate that this design can be adapted to other imaging modalities, enabling the study of samples subject to motion artifacts at higher resolution.
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1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Artefactos
/
Tomografía de Coherencia Óptica
Idioma:
En
Revista:
Opt Lett
Año:
2023
Tipo del documento:
Article