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Overexpression of a nitrate transporter NtNPF2.11 increases nitrogen accumulation and yield in tobacco.
Wu, Xiaoqiu; Zhou, Xiaojie; Wang, Shuaibin; Wang, Zhangying; Huang, Pingjun; Pu, Wenxuan; Peng, Yu; Fan, Xiaorong; Gao, Junping; Li, Zhaowu.
Afiliación
  • Wu X; Puai Medical College, Shaoyang University, Shaoyang 422000, China.
  • Zhou X; College of Food and Chemical Engineering, Shaoyang University, Shaoyang 422000, China.
  • Wang S; Tobacco Research Institute of Technology Centre, China Tobacco Hunan Industrial Corporation, Changsha 410007, China.
  • Wang Z; State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.
  • Huang P; Tobacco Research Institute of Technology Centre, China Tobacco Hunan Industrial Corporation, Changsha 410007, China.
  • Pu W; Tobacco Research Institute of Technology Centre, China Tobacco Hunan Industrial Corporation, Changsha 410007, China.
  • Peng Y; Tobacco Research Institute of Technology Centre, China Tobacco Hunan Industrial Corporation, Changsha 410007, China.
  • Fan X; State Key Laboratory of Crop Genetics and Germplasm Enhancement, MOA Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Nanjing Agricultural University, Nanjing 210095, China.
  • Gao J; Tobacco Research Institute of Technology Centre, China Tobacco Hunan Industrial Corporation, Changsha 410007, China. Electronic address: JunpingGao@163.com.
  • Li Z; Puai Medical College, Shaoyang University, Shaoyang 422000, China. Electronic address: zhaowuli@whu.edu.cn.
Gene ; 885: 147715, 2023 Nov 15.
Article en En | MEDLINE | ID: mdl-37591325
Nitrogen (N) is the key essential macronutrient for crop growth and yield. Over-application of inorganic N fertilizer in fields generated serious environmental pollution and had a negative impact to human health. Therefore, improving crop N use efficiency (NUE) is helpful for sustainable agriculture. The biological functions of nitrogen transporters and regulators have been intensively studied in many crop species. However, only a few nitrogen transporters have been identified in tobacco to date. We reported the identification and functional characterization of a nitrate transporter NtNPF2.11 from tobacco (Nicotiana tabacum). qRT-PCR assay revealed that NtNPF2.11 was mainly expressed in leaf and vein. Under middle N (MN, 1.57 kg N/100 m2) and high N (HN, 2.02 kg N/100 m2) conditions, overexpression of NtNPF2.11 in tobacco greatly improved N utilization and biomass. Moreover, under middle N and high N conditions, the expression of genes for nitrate assimilation, such as NtNR1, NtNiR, NtGS and NtGOGAT, were upregulated in NtNPF2.11 overexpression plants. Compared with WT, overexpression of NtNPF2.11 increased potassium (K) accumulation under high N conditions. These results indicated that overexpression of NtNPF2.11 could increase tobacco yield, N and K accumulation under higher N conditions. Overall, these findings improve our understanding the function of NtNPF2.11 and provide useful gene for sustainable agriculture.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Nicotiana / Transportadores de Nitrato Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Gene Año: 2023 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Nicotiana / Transportadores de Nitrato Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Gene Año: 2023 Tipo del documento: Article País de afiliación: China