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Efficient genome editing in cotton using the virus-mediated CRISPR/Cas9 and grafting system.
Guo, Wei-Feng; Guo, Dan-Dan; Li, Fen; Shang, Shen-Zhai; Li, Ting-Wan; Tang, Ying-Chao; Jiang, Man; Xu, Fu-Chun; Gao, Wei.
Afiliación
  • Guo WF; Agricultural College, Tarim University, Alaer, Xinjiang, People's Republic of China.
  • Guo DD; National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Henan, 475004, People's Republic of China.
  • Li F; National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Henan, 475004, People's Republic of China.
  • Shang SZ; National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Henan, 475004, People's Republic of China.
  • Li TW; National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Henan, 475004, People's Republic of China.
  • Tang YC; National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Henan, 475004, People's Republic of China.
  • Jiang M; National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Henan, 475004, People's Republic of China.
  • Xu FC; Changzhi Medical College, Changzhi, Shanxi, People's Republic of China.
  • Gao W; National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Henan, 475004, People's Republic of China. gaowei021@163.com.
Plant Cell Rep ; 42(11): 1833-1836, 2023 Nov.
Article en En | MEDLINE | ID: mdl-37642675
ABSTRACT
KEY MESSAGE The extensive application of CRISPR in cotton was limited due to the labor-intensive transformation process. Thus, we here established a convenient method of CRISPR in cotton by CLCrV-mediated sgRNA delivery.

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Plant Cell Rep Asunto de la revista: BOTANICA Año: 2023 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Plant Cell Rep Asunto de la revista: BOTANICA Año: 2023 Tipo del documento: Article