Aldo keto-reductase family 1C members 1 through 4 recombinant enzyme purification and enzyme assay.
Methods Enzymol
; 689: 303-329, 2023.
Article
en En
| MEDLINE
| ID: mdl-37802576
ABSTRACT
Aldo-keto reductase family 1C (AKR1C) members transform steroids via their 3-, 17-, and 20-ketosteroid reductase activities. The biochemical study of these enzymes can help to inform their roles in hormone-dependent diseases and develop therapeutic inhibitors. This work describes a protocol to purify AKR1C1-4 members from a bacterial expression system using two chromatography steps. It also describes the basis of discontinuous assays to measure steroid conversion.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Esteroides
/
Pruebas de Enzimas
Tipo de estudio:
Guideline
Idioma:
En
Revista:
Methods Enzymol
Año:
2023
Tipo del documento:
Article