Your browser doesn't support javascript.
loading
Coumarins from Jinhua Finger Citron: Separation by Liquid-Liquid Chromatography and Potential Antitumor Activity.
Wang, Chaoyue; Huang, Jiangang; Zhou, Zhiling; Xu, Ping; Shi, Jingyi; Yang, Yushun; Tong, Shengqiang; Hu, Hongyu.
Afiliación
  • Wang C; Jinhua Advanced Research Institute, Jinhua 321015, China.
  • Huang J; Xingzhi College, Zhejiang Normal University, Lanxi 321100, China.
  • Zhou Z; Xingzhi College, Zhejiang Normal University, Lanxi 321100, China.
  • Xu P; Jinhua Advanced Research Institute, Jinhua 321015, China.
  • Shi J; College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China.
  • Yang Y; Jinhua Advanced Research Institute, Jinhua 321015, China.
  • Tong S; Jinhua Advanced Research Institute, Jinhua 321015, China.
  • Hu H; College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China.
Molecules ; 28(19)2023 Oct 03.
Article en En | MEDLINE | ID: mdl-37836760
ABSTRACT
In this paper, liquid-liquid chromatography was introduced for the first time for the separation of fingered citron (Citrus medica L. var. sarcodactylis Swingle). The fingered citron cultivated in Jinhua is of significant industrial and medicinal value, with several major coumarin compounds detected in its extract. Therefore, further separation for higher purity was of necessity. A preparative liquid-liquid chromatographic method was developed by combining two elution modes (isocratic and step-gradient) with selection according to different polarities of the target sample. Five coumarin derivatives-5,7-dimethoxycoumarin (52.6 mg, 99.6%), phellopterin (4.9 mg, 97.1%), 5-prenyloxy-7-methoxycoumarin (6.7 mg, 98.7%), 6-hydroxy-7-methoxycoumarin (7.1 mg, 82.2%), and byakangelicol (10.5 mg, 90.1%)-with similar structures and properties were isolated on a large scale from 100 mg of petroleum ether (PE) extract and 100 mg of ethyl acetate (EA) extract in Jinhua fingered citron. The productivity was much improved. The anti-growth activity of the isolated coumarins was evaluated against three cancer cell lines (HeLa, A549, and MCF7) with an MTT assay. The coumarins demonstrated potential anti-tumor activity on the HeLa cell line, with 5,7-dimethoxycoumarin in particular exhibiting the best anti-growth activity (IC50 = 10.57 ± 0.24 µM) by inhibiting proliferation. It inhibited colony formation and reduced the size of the tumor sphere in a concentration-dependent manner. The main mechanism was confirmed as inducing apoptosis. This work was informative for further studies aimed at exploring new natural-product-based antitumor agents.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Extractos Vegetales / Citrus Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Extractos Vegetales / Citrus Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: China