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Internal validation of the GA118-24B Genetic Analyzer, a stable capillary electrophoresis system for forensic DNA identification.
Qu, Weifeng; Wang, Feng; Sun, Dan; Liu, Yi; Jin, Xin; Gong, Zheng; Liu, Jinjie; Zhang, Ningjie; Wen, Dan; Wang, Chudong; Jia, Hongtao; Xu, Ruyi; Tang, Xuan; Chen, Siqi; Fu, Xiaoyi; Li, Xue; Rong, Haibo; Zhang, Tao; Jin, Chuan; Chen, Li; Li, Jienan; Liu, Ying; Cai, Jifeng; Jiang, Bowei; Zha, Lagabaiyila.
Afiliación
  • Qu W; Department of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
  • Wang F; Ministry of Public Security of P.R.C., The First Research Institute, No. 1. Shouti South Road, Beijing, 100044, China.
  • Sun D; Ministry of Public Security of P.R.C., The First Research Institute, No. 1. Shouti South Road, Beijing, 100044, China.
  • Liu Y; Department of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
  • Jin X; Public Security Department of Hainan Province, No. 9. Binya Road, Hainan, 570203, China.
  • Gong Z; Criminal Technology Detachment, Public Security Bureau of Harbin, No. 35. Dongfeng Road, Harbin, 150010, China.
  • Liu J; Criminal Investigation Detachment, Public Security Bureau of Beijing, No. 44. Banbuqiao Road, Beijing, 102611, China.
  • Zhang N; Ministry of Public Security of P.R.C., The First Research Institute, No. 1. Shouti South Road, Beijing, 100044, China.
  • Wen D; Department of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
  • Wang C; Department of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
  • Jia H; Department of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
  • Xu R; Department of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
  • Tang X; Department of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
  • Chen S; Department of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
  • Fu X; Department of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
  • Li X; Department of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
  • Rong H; Ministry of Public Security of P.R.C., The First Research Institute, No. 1. Shouti South Road, Beijing, 100044, China.
  • Zhang T; Ministry of Public Security of P.R.C., The First Research Institute, No. 1. Shouti South Road, Beijing, 100044, China.
  • Jin C; Ministry of Public Security of P.R.C., The First Research Institute, No. 1. Shouti South Road, Beijing, 100044, China.
  • Chen L; Ministry of Public Security of P.R.C., The First Research Institute, No. 1. Shouti South Road, Beijing, 100044, China.
  • Li J; Department of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
  • Liu Y; Department of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
  • Cai J; Department of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China.
  • Jiang B; Ministry of Public Security of P.R.C., The First Research Institute, No. 1. Shouti South Road, Beijing, 100044, China. jiangbw@fiscan.cn.
  • Zha L; Department of Forensic Science, School of Basic Medical Sciences, Central South University, No. 172. Tongzipo Road, Changsha, 410013, China. dlkl2001@163.com.
Int J Legal Med ; 138(2): 361-373, 2024 Mar.
Article en En | MEDLINE | ID: mdl-37843624
ABSTRACT
The GA118-24B Genetic Analyzer (hereafter, "GA118-24B") is an independently developed capillary electrophoresis instrument. In the present research, we designed a series of validation experiments to test its performance at detecting DNA fragments compared to the Applied Biosystems 3500 Genetic Analyzer (hereafter, "3500"). Three commercially available autosomal short tandem repeat multiplex kits were used in this validation. The results showed that GA118-24B had acceptable spectral calibration for three kits. The results of accuracy and concordance studies were also satisfactory. GA118-24B showed excellent precision, with a standard deviation of less than 0.1 bp. Sensitivity and mixture studies indicated that GA118-24B could detect low-template DNA and complex mixtures as well as the results generated by 3500 in parallel experiments. Based on the experimental results, we set specific analytical and stochastic thresholds. Besides, GA118-24B showed superiority than 3500 within certain size ranges in the resolution study. Instead of conventional commercial multiplex kits, GA118-24B performed stably on a self-developed eight-dye multiplex system, which were not performed on 3500 Genetic Analyzer. We compared our validation results with those of previous research and found our results to be convincing. Overall, we conclude that GA118-24B is a stable and reliable genetic analyzer for forensic DNA identification.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: ADN / Dermatoglifia del ADN Límite: Humans Idioma: En Revista: Int J Legal Med Asunto de la revista: JURISPRUDENCIA Año: 2024 Tipo del documento: Article País de afiliación: China
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: ADN / Dermatoglifia del ADN Límite: Humans Idioma: En Revista: Int J Legal Med Asunto de la revista: JURISPRUDENCIA Año: 2024 Tipo del documento: Article País de afiliación: China