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A macrocyclic fluorescent probe for the detection of citrate.
Butler, Stephen M; Hountondji, Maria; Berry, Stuart N; Tan, Jian; Macia, Laurence; Jolliffe, Katrina A.
Afiliación
  • Butler SM; School of Chemistry, The University of Sydney, NSW, 2006, Australia. kate.jolliffe@sydney.edu.au.
  • Hountondji M; Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Sydney, 2006, NSW, Australia.
  • Berry SN; School of Chemistry, The University of Sydney, NSW, 2006, Australia. kate.jolliffe@sydney.edu.au.
  • Tan J; Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Sydney, 2006, NSW, Australia.
  • Macia L; School of Chemistry, The University of Sydney, NSW, 2006, Australia. kate.jolliffe@sydney.edu.au.
  • Jolliffe KA; The Charles Perkins Centre, The University of Sydney, NSW 2006, Australia.
Org Biomol Chem ; 21(42): 8548-8553, 2023 11 01.
Article en En | MEDLINE | ID: mdl-37846461
ABSTRACT
We present a macrocyclic fluorescent probe for the detection of citrate. This receptor binds citrate through hydrogen-bonding interactions in aqueous solutions, and exhibits a turn-on in fluorescence in response to binding. The presence of common biologically relevant dicarboxylate species does not significantly impact the fluorescence response. We have demonstrated the utility of this probe with the staining of murine splenocytes, and identified different basal levels of citrate present in immune cell subsets via flow cytometry analysis.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ácido Cítrico / Colorantes Fluorescentes Límite: Animals Idioma: En Revista: Org Biomol Chem Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Australia
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ácido Cítrico / Colorantes Fluorescentes Límite: Animals Idioma: En Revista: Org Biomol Chem Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Australia