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Disorder and amino acid composition in proteins: their potential role in the adaptation of extracellular pilins to the acidic media, where Acidithiobacillus thiooxidans grows.
Páez-Pérez, Edgar D; Hernández-Sánchez, Araceli; Alfaro-Saldaña, Elvia; García-Meza, J Viridiana.
Afiliación
  • Páez-Pérez ED; Geomicrobiología, Metalurgia, Universidad Autónoma de San Luis Potosí, Sierra Leona 550, 78210, San Luis Potosí, SLP, Mexico. edpaezperez@gmail.com.
  • Hernández-Sánchez A; Geomicrobiología, Metalurgia, Universidad Autónoma de San Luis Potosí, Sierra Leona 550, 78210, San Luis Potosí, SLP, Mexico. aracelihersan@gmail.com.
  • Alfaro-Saldaña E; Geomicrobiología, Metalurgia, Universidad Autónoma de San Luis Potosí, Sierra Leona 550, 78210, San Luis Potosí, SLP, Mexico.
  • García-Meza JV; Geomicrobiología, Metalurgia, Universidad Autónoma de San Luis Potosí, Sierra Leona 550, 78210, San Luis Potosí, SLP, Mexico.
Extremophiles ; 27(3): 31, 2023 Oct 17.
Article en En | MEDLINE | ID: mdl-37848738
ABSTRACT
There are few biophysical studies or structural characterizations of the type IV pilin system of extremophile bacteria, such as the acidophilic Acidithiobacillus thiooxidans. We set out to analyze their pili-comprising proteins, pilins, because these extracellular proteins are in constant interaction with protons of the acidic medium in which At. thiooxidans grows. We used the web server Operon Mapper to analyze and identify the cluster codified by the minor pilin of At. thiooxidans. In addition, we carried an in-silico characterization of such pilins using the VL-XT algorithm of PONDR® server. Our results showed that structural disorder prevails more in pilins of At. thiooxidans than in non-acidophilic bacteria. Further computational characterization showed that the pilins of At. thiooxidans are significantly enriched in hydroxy (serine and threonine) and amide (glutamine and asparagine) residues, and significantly reduced in charged residues (aspartic acid, glutamic acid, arginine and lysine). Similar results were obtained when comparing pilins from other Acidithiobacillus and other acidophilic bacteria from another genus versus neutrophilic bacteria, suggesting that these properties are intrinsic to pilins from acidic environments, most likely by maintaining solubility and stability in harsh conditions. These results give guidelines for the application of extracellular proteins of acidophiles in protein engineering.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Fimbrias / Acidithiobacillus Idioma: En Revista: Extremophiles Asunto de la revista: BIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: México

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Fimbrias / Acidithiobacillus Idioma: En Revista: Extremophiles Asunto de la revista: BIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: México