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Nucleotide at position 66 of NS2A in Japanese encephalitis virus is associated with the virulence and proliferation of virus.
Tan, Ning; Chen, Chen; Ren, Yang; Huang, Rong; Zhu, Zhuang; Xu, Kui; Yang, Xiaoyao; Yang, Jian; Yuan, Lei.
Afiliación
  • Tan N; Department of Pathogenic Biology, School of Basic Medical Sciences and Forensic Medicine, North Sichuan Medical College, Nanchong, 637100, China.
  • Chen C; Department of Pathogenic Biology, School of Basic Medical Sciences and Forensic Medicine, North Sichuan Medical College, Nanchong, 637100, China.
  • Ren Y; Department of Laboratory Medicine, Jintang First People's Hospital, West China Hospital Sichuan University JinTang Hospital, Chengdu, 610400, China.
  • Huang R; Department of Pathogenic Biology, School of Basic Medical Sciences and Forensic Medicine, North Sichuan Medical College, Nanchong, 637100, China.
  • Zhu Z; Department of Pathogenic Biology, School of Basic Medical Sciences and Forensic Medicine, North Sichuan Medical College, Nanchong, 637100, China.
  • Xu K; Department of Pathogenic Biology, School of Basic Medical Sciences and Forensic Medicine, North Sichuan Medical College, Nanchong, 637100, China.
  • Yang X; Department of Pathogenic Biology, School of Basic Medical Sciences and Forensic Medicine, North Sichuan Medical College, Nanchong, 637100, China.
  • Yang J; Department of Pathogenic Biology, School of Basic Medical Sciences and Forensic Medicine, North Sichuan Medical College, Nanchong, 637100, China.
  • Yuan L; Department of Pathogenic Biology, School of Basic Medical Sciences and Forensic Medicine, North Sichuan Medical College, Nanchong, 637100, China. small7@126.com.
Virus Genes ; 60(1): 9-17, 2024 Feb.
Article en En | MEDLINE | ID: mdl-37938470
ABSTRACT
Most wild strains of Japanese encephalitis virus (JEV) produce NS1' protein, which plays an important role in viral infection and immune escape. The G66A nucleotide mutation in NS2A gene of the wild strain SA14 prevented the ribosomal frameshift that prevented the production of NS1' protein, thus reduced the virulence. In this study, the 66th nucleotide of the NS2A gene of SA14 was mutated into A, U or C, respectively. Both the G66U and G66C mutations cause the E22D mutation of the NS2A protein. Subsequently, the expression of NS1' protein, plaque size, replication ability, and virulence to mice of the three mutant strains were examined. The results showed that the three mutant viruses could not express NS1' protein, and their proliferation ability in nerve cells and virulence to mice were significantly reduced. In addition, the SA14(G66C) was less virulent than the other two mutated viruses. Our results indicate that only when G is the 66th nucleotide of NS2A, the JEV can produce NS1' protein, which affects the virulence.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Encefalitis Japonesa / Virus de la Encefalitis Japonesa (Especie) Límite: Animals Idioma: En Revista: Virus Genes Asunto de la revista: BIOLOGIA MOLECULAR / VIROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Encefalitis Japonesa / Virus de la Encefalitis Japonesa (Especie) Límite: Animals Idioma: En Revista: Virus Genes Asunto de la revista: BIOLOGIA MOLECULAR / VIROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China