Protocol for performing and optimizing differential scanning fluorimetry experiments.
STAR Protoc
; 4(4): 102688, 2023 Dec 15.
Article
en En
| MEDLINE
| ID: mdl-37943662
ABSTRACT
Differential scanning fluorimetry (DSF) is a widely used technique for determining the apparent melting temperature (Tma) of a purified protein. Here, we present a protocol for performing and optimizing DSF experiments. We describe steps for designing and performing the experiment, analyzing data, and optimization. We provide benchmarks for typical Tmas and ΔTmas, standard assay conditions, and upper and lower limits of commonly altered experimental variables. We also detail common pitfalls of DSF and ways to avoid, identify, and overcome them.
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1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas
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Idioma:
En
Revista:
STAR Protoc
Año:
2023
Tipo del documento:
Article
País de afiliación:
Estados Unidos