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In Vitro Evaluation of Vancomycin-Induced Toxicity in Human Primary Knee Chondrocytes.
Hall, Susan; Grayson, Jane; Grant, Gary; Vertullo, Christopher; Anoopkumar-Dukie, Shailendra.
Afiliación
  • Hall S; School of Pharmacy and Medical Sciences, Griffith University, Gold Coast, Australia.
  • Grayson J; School of Health Sciences, The University of Sydney, Glebe, Australia.
  • Grant G; School of Pharmacy and Medical Sciences, Griffith University, Gold Coast, Australia.
  • Vertullo C; The Knee and Sports Medicine Centre, Gold Coast, Australia.
  • Anoopkumar-Dukie S; School of Pharmacy and Medical Sciences, Griffith University, Gold Coast, Australia.
Int J Toxicol ; 43(2): 177-183, 2024.
Article en En | MEDLINE | ID: mdl-37989131
Septic arthritis as a complication of orthopaedic joint surgery can have catastrophic outcomes for patients. To minimise infection risk associated with elective orthopaedics, topical vancomycin during surgery has become increasingly common. Evidence suggests that high concentrations of vancomycin, following direct application of the drug to the joint, are toxic towards various local cell types in the joint, including chondrocytes. However, the mechanism of this vancomycin tissue toxicity is yet to be determined. The aim of this study was to evaluate the toxicity of vancomycin on chondrocytes and the mechanisms of cell death involved. Human primary knee chondrocytes were exposed to vancomycin (1.25-10 mg/mL) for 24 h and their viability assessed using the resazurin reduction assay in vitro. Specific cell death mechanisms and their contributors, including reactive oxygen species (ROS) production and apoptosis, were measured. This study showed that high concentrations of vancomycin (5 and 10 mg/mL) were toxic towards human primary knee chondrocyte cells, while lower concentrations (1.25 and 2.5 mg/mL) were not. Cell death studies found that this occurred through an apoptotic pathway. This study provides additional support that vancomycin in high doses is toxic towards chondrocytes and preliminary evidence that this toxicity occurs via apoptotic cell death mechanisms.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Vancomicina / Condrocitos Límite: Humans Idioma: En Revista: Int J Toxicol Asunto de la revista: TOXICOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Australia

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Vancomicina / Condrocitos Límite: Humans Idioma: En Revista: Int J Toxicol Asunto de la revista: TOXICOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Australia