Estimation of the reference values and decision limits for growth hormone in newborns using dried blood spots.
Clin Chem Lab Med
; 62(5): 939-945, 2024 Apr 25.
Article
en En
| MEDLINE
| ID: mdl-37999718
ABSTRACT
OBJECTIVES:
Severe deficiency of growth hormone (GHD) of the newborn is a rare but potentially life-threatening disease. GH measured during the first week of life, using dried blood spots (DBS), may offer several advantages. Aim of the study was to estimate the reference values for GH in newborns by a new analytical method using DBS.METHODS:
Using a new developed analytical method, GH was estimated from DBS of 1,036 healthy newborns attending the Neonatology Unit of Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico of Milan in the period July-October 2021. Reference values for GH deficiency were estimated by the Harrell-Davis bootstrap method, with 90â¯%CI calculated by the bias-corrected and accelerated bootstrap method.RESULTS:
All GH measurements required 33 analytical sessions (8 months) with a CV% for calibration curve slopes equal to 6.9â¯%. Intermediate precision evaluated by measurement of low (3⯵g/L) and high (10⯵g/L) quality controls was, respectively, 14 and 6.5â¯%. GH reference values, estimated at percentiles 1.0st, 2.5th and 5.0th, and their 90â¯%CI, were, respectively, 4.5⯵g/L (90â¯%CI 3.8-5.1), 5.9⯵g/L (90â¯%CI 5.4-6.4) and 7.0⯵g/L (90â¯%CI 6.7-7.3). GH levels were not associated with sex, standard deviation scores, birth weight, gestational age, type of delivery or mother's variables (age, smoking habit, gestational diabetes).CONCLUSIONS:
Validation data suggest that this method can be used to measured GH in newborns using DBS. The reference values estimated in this study are in accordance with previous published works using ELISA and may help confirming the clinical suspicion of neonatal GHD.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Hormona del Crecimiento
/
Hormona de Crecimiento Humana
Límite:
Humans
/
Newborn
Idioma:
En
Revista:
Clin Chem Lab Med
Asunto de la revista:
QUIMICA CLINICA
/
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2024
Tipo del documento:
Article
País de afiliación:
Italia