Your browser doesn't support javascript.
loading
Evaluation of the expression of LC3-II and BECLIN1 genes of autophagy pathway in patients with hematological malignancies.
Ayatollahi, Hossein; Boroumand-Noughabi, Samaneh; Ferns, Gordon; Sheikhi, Maryam; Siyadat, Payam; Rostami, Mehrdad; Khoshnegah, Zahra.
Afiliación
  • Ayatollahi H; Department of Hematology and Blood Banking, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Boroumand-Noughabi S; Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Ferns G; Department of Hematology and Blood Banking, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Sheikhi M; Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Siyadat P; Department of Medical Education, Brighton and Sussex Medical School, Brighton, UK.
  • Rostami M; Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Khoshnegah Z; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
Caspian J Intern Med ; 14(4): 694-702, 2023.
Article en En | MEDLINE | ID: mdl-38024167
ABSTRACT

Background:

Autophagy is a pathway for the degradation of cytoplasmic components, which plays an essential role in various cellular and physiological processes, including cell renewal and survival, and immune responses. While recent studies have shown that they can play a role in cancer treatment, the precise mechanisms of autophagy in leukemogenesis are not fully understood. We have assessed the expression levels of LC3 and BECLIN1 as two crucial autophagy mediators in patients with leukemia.

Methods:

This cross-sectional study was performed on bone marrow or peripheral blood samples of 61 leukemia patients (24 AML, 20 ALL, and 17 CML) and compared to 18 healthy controls. Real-time PCR was used to quantitate gene expression. SPSS statistics 16.0 and Graph Pad Prism 8.4.2 software were applied for statistical analysis.

Results:

While BECLIN1 expression was significantly lower in AML, ALL, and CML patients as compared to the control group (p < 0.05), LC3 showed significantly different expression only in the AML patients (P= 0.03). There was no significant correlation between the expression levels of BECLIN1 with LC3 (p> 0.05). Whilst the AML LC3high group had a significantly lower lymphocyte count (P= 0.023), the AML BECLIN1low group had a significantly higher MPV levels (P= 0.044). Furthermore, ALL LC3high group indicated a significantly lower HCT count (P= 0.017).

Conclusion:

Significant changes in the expression levels of BECLINI and LC3 in hematologic malignancies may indicate a possible role for autophagy in their pathogenesis. However, further studies are warranted to confirm these findings.
Palabras clave

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Caspian J Intern Med Año: 2023 Tipo del documento: Article País de afiliación: Irán

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Caspian J Intern Med Año: 2023 Tipo del documento: Article País de afiliación: Irán