The mating pilus of E. coli pED208 acts as a conduit for ssDNA during horizontal gene transfer.
mBio
; 15(1): e0285723, 2024 Jan 16.
Article
en En
| MEDLINE
| ID: mdl-38051116
ABSTRACT
IMPORTANCE Bacteria are constantly exchanging DNA, which constitutes horizontal gene transfer. While some of these occurs by a non-specific process called natural transformation, some occurs by a specific mating between a donor and a recipient cell. In specific conjugation, the mating pilus is extended from the donor cell to make contact with the recipient cell, but whether DNA is actually transferred through this pilus or by another mechanism involving the type IV secretion system complex without the pilus has been an open question. Using Escherichia coli, we show that DNA can be transferred through this pilus between a donor and a recipient cell that has not established a tight mating junction, providing a new picture for the role of this pilus.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Transferencia de Gen Horizontal
/
Escherichia coli
Idioma:
En
Revista:
MBio
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos