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Discovery and characterization of genes conferring natural resistance to the antituberculosis antibiotic capreomycin.
Toh, Shu-Ing; Elaine Keisha, Johan; Wang, Yung-Lin; Pan, Yi-Chi; Jhu, Yu-Heng; Hsiao, Po-Yun; Liao, Wen-Ting; Chen, Po-Yuan; Ko, Tai-Ming; Chang, Chin-Yuan.
Afiliación
  • Toh SI; Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30010, Taiwan, ROC.
  • Elaine Keisha J; Institute of Molecular Medicine and Bioengineering, National Yang Ming Chiao Tung University, Hsinchu, 30010, Taiwan, ROC.
  • Wang YL; Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30010, Taiwan, ROC.
  • Pan YC; Genomics Research Center, Academia Sinica, Taipei, 11529, Taiwan, ROC.
  • Jhu YH; Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30010, Taiwan, ROC.
  • Hsiao PY; Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30010, Taiwan, ROC.
  • Liao WT; Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30010, Taiwan, ROC.
  • Chen PY; Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30010, Taiwan, ROC.
  • Ko TM; Institute of Molecular Medicine and Bioengineering, National Yang Ming Chiao Tung University, Hsinchu, 30010, Taiwan, ROC.
  • Chang CY; Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30010, Taiwan, ROC.
Commun Biol ; 6(1): 1282, 2023 12 19.
Article en En | MEDLINE | ID: mdl-38114770
ABSTRACT
Metagenomic-based studies have predicted an extraordinary number of potential antibiotic-resistance genes (ARGs). These ARGs are hidden in various environmental bacteria and may become a latent crisis for antibiotic therapy via horizontal gene transfer. In this study, we focus on a resistance gene cph, which encodes a phosphotransferase (Cph) that confers resistance to the antituberculosis drug capreomycin (CMN). Sequence Similarity Network (SSN) analysis classified 353 Cph homologues into five major clusters, where the proteins in cluster I were found in a broad range of actinobacteria. We examine the function and antibiotics targeted by three putative resistance proteins in cluster I via biochemical and protein structural analysis. Our findings reveal that these three proteins in cluster I confer resistance to CMN, highlighting an important aspect of CMN resistance within this gene family. This study contributes towards understanding the sequence-structure-function relationships of the phosphorylation resistance genes that confer resistance to CMN.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Capreomicina / Antibacterianos Idioma: En Revista: Commun Biol Año: 2023 Tipo del documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Capreomicina / Antibacterianos Idioma: En Revista: Commun Biol Año: 2023 Tipo del documento: Article