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Discovery and verification of extracellular microRNA biomarkers for diagnostic and prognostic assessment of preeclampsia at triage.
Morey, Robert; Poling, Lara; Srinivasan, Srimeenakshi; Martinez-King, Carolina; Anyikam, Adanna; Zhang-Rutledge, Kathy; To, Cuong; Hakim, Abbas; Mochizuki, Marina; Verma, Kajal; Mason, Antoinette; Tran, Vy; Meads, Morgan; Lamale-Smith, Leah; Roeder, Hilary; Horii, Mariko; Ramos, Gladys A; DeHoff, Peter; Parast, Mana M; Pantham, Priyadarshini; Laurent, Louise C.
Afiliación
  • Morey R; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • Poling L; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • Srinivasan S; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • Martinez-King C; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • Anyikam A; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • Zhang-Rutledge K; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • To C; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • Hakim A; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • Mochizuki M; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • Verma K; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • Mason A; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • Tran V; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • Meads M; Department of Pathology, University of California, San Diego, La Jolla, CA, USA.
  • Lamale-Smith L; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • Roeder H; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • Horii M; Department of Pathology, University of California, San Diego, La Jolla, CA, USA.
  • Ramos GA; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • DeHoff P; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • Parast MM; Department of Pathology, University of California, San Diego, La Jolla, CA, USA.
  • Pantham P; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
  • Laurent LC; Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA.
Sci Adv ; 9(51): eadg7545, 2023 Dec 22.
Article en En | MEDLINE | ID: mdl-38117879
ABSTRACT
We report on the identification of extracellular miRNA (ex-miRNA) biomarkers for early diagnosis and prognosis of preeclampsia (PE). Small RNA sequencing of maternal serum prospectively collected from participants undergoing evaluation for suspected PE revealed distinct patterns of ex-miRNA expression among different categories of hypertensive disorders in pregnancy. Applying an iterative machine learning method identified three bivariate miRNA biomarkers (miR-522-3p/miR-4732-5p, miR-516a-5p/miR-144-3p, and miR-27b-3p/let-7b-5p) that, when applied serially, distinguished between PE cases of different severity and differentiated cases from controls with a sensitivity of 93%, specificity of 79%, positive predictive value (PPV) of 55%, and negative predictive value (NPV) of 89%. In a small independent validation cohort, these ex-miRNA biomarkers had a sensitivity of 91% and specificity of 57%. Combining these ex-miRNA biomarkers with the established sFlt1PlGF protein biomarker ratio performed better than either set of biomarkers alone (sensitivity of 89.4%, specificity of 91.3%, PPV of 95.5%, and NPV of 80.8%).
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Preeclampsia / MicroARNs Límite: Female / Humans / Pregnancy Idioma: En Revista: Sci Adv Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Preeclampsia / MicroARNs Límite: Female / Humans / Pregnancy Idioma: En Revista: Sci Adv Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos