Treatment of ovarian damage induced by chemotherapeutic drugs in female rats with G-CSF and platelet-rich plasma(PRP): an immunohistochemical study correlation with novel marker INSL-3.
Gynecol Endocrinol
; 40(1): 2301551, 2024 Dec.
Article
en En
| MEDLINE
| ID: mdl-38195404
ABSTRACT
OBJECTIVE:
To assess the impacts of Platelet-Rich Plasma(PRP) and Granulocyte Colony-Stimulating Factor(G-CSF) on a rat model with induced ovarian follicular damage caused by cyclophosphamide(Cy). MATERIALS ANDMETHODS:
Forty-two Sprague-Dawley rats were randomly allocated into seven distinct groups as; Group 1(control) NaCl intraperitoneal (IP) injection was administered on days D1, D7, and D14. Group 2(Cy)Cy IP injection on D1 + NaCl IP injection on D7 and D14 were administered. Group 3(PRP) PRP IP injection on D1,D7 and D14 were administered. Group 4(Cy + PRP)Cy IP injection on D1 and PRP IP injection on D1, D7 and D14 were administered. Group 5(G-CSF) G-CSF IP injection on D1, D7 and D14 were administered. Group 6(Cy + G-CSF)Cy IP injection on D1+ G-CSF IP injection on D1, D7 and D14 were administered. Group 7(Cy + PRP + G-CSF)Cy IP injection on D1+ PRP IP injection on D1,D7 and D14+ G-CSF IP injection on D1,D7 and D14 were administered. Follicular number, histological scores of AMH and INSL3 stained follicles at different stages of follicular development, and serum Anti-Müllerian hormone(AMH) were evaluated.RESULTS:
The primary, secondary, and antral follicle intensity scores for AMH-positive staining were most prominent in Groups 3 and 5. There was no significant difference between groups 4, 6 and 7 compared to group 1 in terms of follicule counts and AMH staining. The intensity scores of AMH-positive staining follicles were notably reduced in group 2 compared to groups 4, 6, and 7, with a significant difference (p < .01). Among the groups, group 2 exhibited the least intense antral follicle staining for INSL3, displaying a significant difference(p < .01) compared to the remaining groups.CONCLUSIONS:
Autologous PRP and G-CSF might protect ovarian function in the face of ovarian damage caused by Cy-induced effects.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Hormonas Peptídicas
/
Plasma Rico en Plaquetas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Gynecol Endocrinol
Asunto de la revista:
ENDOCRINOLOGIA
/
GINECOLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Turquía