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Wnt3a Facilitates SARS-CoV-2 Pseudovirus Entry into Cells.
Melano, Ivonne; Chen, Hui-Jye; Ngwira, Loveness; Hsu, Pang-Hung; Kuo, Li-Lan; Noriega, Lloyd; Su, Wen-Chi.
Afiliación
  • Melano I; Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404, Taiwan.
  • Chen HJ; Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404, Taiwan.
  • Ngwira L; International Master's Program of Biomedical Sciences, China Medical University, Taichung 404, Taiwan.
  • Hsu PH; Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 202, Taiwan.
  • Kuo LL; Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 202, Taiwan.
  • Noriega L; Institute of Biochemistry and Molecular Biology, National Yang Ming Chiao Tung University, Taipei 112, Taiwan.
  • Su WC; Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404, Taiwan.
Int J Mol Sci ; 25(1)2023 Dec 22.
Article en En | MEDLINE | ID: mdl-38203386
ABSTRACT
How ACE2 functions as the major host receptor of SARS-CoV-2 despite having low expression in the lungs is still unknown. To facilitate the development of therapeutic strategies against coronaviruses, gaining a deeper comprehension of the molecular mechanism of SARS-CoV-2 infection is imperative. In our previous study, we identified several potential host factors of SARS-CoV-2 using an shRNA arrayed screen, one of which was Wnt3a. Here, we validated the significance of Wnt3a, a potent activator of the Wnt/ß-catenin signaling pathway, for SARS-CoV-2 entry into cells by evaluating the effects of its knockdown and overexpression on SARS-CoV-2 pseudotyped virus entry. Further analysis revealed that SARS-CoV-2 pseudotyped virus infection activates the canonical Wnt/ß-catenin signaling pathway, which we found could subsequently stimulate ACE2 transcription. Collectively, our study identified Wnt3a as an important host factor that facilitates ACE2-mediated virus infection. Insight into the virus entry mechanism is impactful as it will aid in developing novel therapeutic strategies against current and future coronavirus pandemics.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2023 Tipo del documento: Article País de afiliación: Taiwán

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2023 Tipo del documento: Article País de afiliación: Taiwán