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Antibody Response to the Sneathia vaginalis Cytopathogenic Toxin A during Pregnancy.
McCoy, Zion T; Serrano, Myrna G; Edupuganti, Laahirie; Spaine, Katherine M; Edwards, David J; Buck, Gregory A; Jefferson, Kimberly K.
Afiliación
  • McCoy ZT; Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA.
  • Serrano MG; Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA.
  • Edupuganti L; Center for Microbiome Engineering and Data Analysis, Virginia Commonwealth University, Richmond, VA.
  • Spaine KM; Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA.
  • Edwards DJ; Center for Microbiome Engineering and Data Analysis, Virginia Commonwealth University, Richmond, VA.
  • Buck GA; Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA.
  • Jefferson KK; Center for Microbiome Engineering and Data Analysis, Virginia Commonwealth University, Richmond, VA.
Immunohorizons ; 8(1): 114-121, 2024 Jan 01.
Article en En | MEDLINE | ID: mdl-38276916
ABSTRACT
Sneathia vaginalis is a Gram-negative vaginal species that is associated with pregnancy complications. It produces cytopathogenic toxin A (CptA), a pore-forming toxin. To determine whether CptA is expressed in vivo and to examine the mucosal Ab response to the toxin, we examined human midvaginal swab samples obtained during pregnancy for IgM, IgA, and IgG Abs with CptA affinity. This subcohort study included samples from 93 pregnant people. S. vaginalis relative abundance was available through 16S rRNA survey. There were 22 samples from pregnancies that resulted in preterm birth in which S. vaginalis relative abundance was <0.005%, 22 samples from pregnancies that resulted in preterm birth with S. vaginalis ≥0.005%, 24 samples from pregnancies that resulted in term birth with S. vaginalis <0.005%, and 25 samples from pregnancies that resulted in term birth with S. vaginalis ≥0.005%. IgM, IgA, and IgG with affinity for CptA were assessed by ELISA. The capacity for the samples to neutralize CptA was quantified by hemolysis assay. All three Ab isotypes were detectable within different subsets of the samples. There was no significant association between relative abundance of S. vaginalis and the presence of any Ab isotype. The majority of vaginal swab samples containing detectable levels of anti-CptA Abs neutralized the hemolytic activity of CptA, with the strongest correlation between IgA and neutralizing activity. These results demonstrate that S. vaginalis produces CptA in vivo and that CptA is recognized by the host immune defenses, resulting in the production of Abs with toxin-neutralizing ability.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Nacimiento Prematuro / Etilaminas Límite: Female / Humans / Newborn / Pregnancy Idioma: En Revista: ImmunoHorizons / Immunohorizons Año: 2024 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Nacimiento Prematuro / Etilaminas Límite: Female / Humans / Newborn / Pregnancy Idioma: En Revista: ImmunoHorizons / Immunohorizons Año: 2024 Tipo del documento: Article