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Microtubule-binding protein MAP1B regulates interstitial axon branching of cortical neurons via the tubulin tyrosination cycle.
Ziak, Jakub; Dorskind, Joelle M; Trigg, Brian; Sudarsanam, Sriram; Jin, Xinyu O; Hand, Randal A; Kolodkin, Alex L.
Afiliación
  • Ziak J; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins Kavli Neuroscience Discovery Institute, The Johns Hopkins School of Medicine, 725 North Wolfe St., Baltimore, MD, 21205, USA.
  • Dorskind JM; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins Kavli Neuroscience Discovery Institute, The Johns Hopkins School of Medicine, 725 North Wolfe St., Baltimore, MD, 21205, USA.
  • Trigg B; Novartis Institutes for BioMedical Research, Boston, MA, USA.
  • Sudarsanam S; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins Kavli Neuroscience Discovery Institute, The Johns Hopkins School of Medicine, 725 North Wolfe St., Baltimore, MD, 21205, USA.
  • Jin XO; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins Kavli Neuroscience Discovery Institute, The Johns Hopkins School of Medicine, 725 North Wolfe St., Baltimore, MD, 21205, USA.
  • Hand RA; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins Kavli Neuroscience Discovery Institute, The Johns Hopkins School of Medicine, 725 North Wolfe St., Baltimore, MD, 21205, USA.
  • Kolodkin AL; The Solomon H. Snyder Department of Neuroscience, Johns Hopkins Kavli Neuroscience Discovery Institute, The Johns Hopkins School of Medicine, 725 North Wolfe St., Baltimore, MD, 21205, USA.
EMBO J ; 43(7): 1214-1243, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38388748
ABSTRACT
Regulation of directed axon guidance and branching during development is essential for the generation of neuronal networks. However, the molecular mechanisms that underlie interstitial (or collateral) axon branching in the mammalian brain remain unresolved. Here, we investigate interstitial axon branching in vivo using an approach for precise labeling of layer 2/3 callosal projection neurons (CPNs). This method allows for quantitative analysis of axonal morphology at high acuity and also manipulation of gene expression in well-defined temporal windows. We find that the GSK3ß serine/threonine kinase promotes interstitial axon branching in layer 2/3 CPNs by releasing MAP1B-mediated inhibition of axon branching. Further, we find that the tubulin tyrosination cycle is a key downstream component of GSK3ß/MAP1B signaling. These data suggest a cell-autonomous molecular regulation of cortical neuron axon morphology, in which GSK3ß can release a MAP1B-mediated brake on interstitial axon branching upstream of the posttranslational tubulin code.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Tubulina (Proteína) / Proteínas Portadoras Límite: Animals Idioma: En Revista: EMBO J Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Tubulina (Proteína) / Proteínas Portadoras Límite: Animals Idioma: En Revista: EMBO J Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos