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Surface enhanced Raman spectroscopy for the characterization of the metabolites of the biodesulfurization of dibenzothiophene carried out by Tsukamurella paurometabola.
Habiba, Umm E; Anwer, Ayesha; Hussain, Muhammad Umar; Majeed, Muhammad Irfan; Alwadie, Najah; Nawaz, Haq; Akhtar, Nasrin; Rashid, Nosheen; Nadeem, Sana; Naz, Maira; Shahzadi, Aqsa; Shehnaz, Hina; Imran, Muhammad.
Afiliación
  • Habiba UE; Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan.
  • Anwer A; Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan.
  • Hussain MU; Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan.
  • Majeed MI; Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan. Electronic address: irfan.majeed@uaf.edu.pk.
  • Alwadie N; Department of Physics, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia. Electronic address: nhalwadie@pnu.edu.sa.
  • Nawaz H; Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan.
  • Akhtar N; Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan. Electronic address: nasrin@nibge.org.
  • Rashid N; Department of Chemistry, University of Education, Faisalabad Campus, Faisalabad 38000, Pakistan.
  • Nadeem S; Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan.
  • Naz M; Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan.
  • Shahzadi A; Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan.
  • Shehnaz H; Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan.
  • Imran M; Department of Chemistry, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia.
Spectrochim Acta A Mol Biomol Spectrosc ; 313: 124126, 2024 May 15.
Article en En | MEDLINE | ID: mdl-38490122
ABSTRACT
Large amount of sulphur is released by the combustion of fossil fuels in the form of SoX which affects human health and leads to acid rain. To overcome this issue, it is essential to eliminate sulphur moieties from heterocyclic organo-sulphur compounds like Dibenzothiophene (DBT) present in the petrol. In this study Surface enhanced Raman scattering (SERS) spectroscopy is used to analyze the desulfurizing activity of Tsukamurella paurometabola bacterial strain. The most prominent SERS peaks observed at 791, 837, 944 and 1032 cm-1, associated to C-S stretching, are solely observed in dibenzothiophene and its metabolite-I (DBTS) but absent in 2-Hydroxybiphenyl (metabolite-II) and extraction sample of supernatant as a result of biodesulfurization. Moreover, the SERS peaks observed at 974 (characteristic peak of benzene ring) and 1015 cm-1 is associated to C-C ring breathing while 1642 and 1655 cm-1 assigned to CC bonds of aromatic ring. These peaks are only observed in 2-Hydroxybiphenyl (metabolite-II) and extraction sample of supernatant as a result of biodesulfurization. Notably, these peaks are absent in the Dibenzothiophene and its metabolite-I which indicate that aromatic ring is carrying sulfur in this fraction. Moreover, multivariate data analytical tools like principal component analysis (PCA) and PCA-loadings are applied to further differentiate between dibenzothiophene and its metabolites that are Dibenzothiophene sulphone (metabolite-I) and 2-Hydroxybiphenyl (metabolite-II).
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Espectrometría Raman / Azufre / Tiofenos / Compuestos de Bifenilo / Actinobacteria Límite: Humans Idioma: En Revista: Spectrochim Acta A Mol Biomol Spectrosc Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Pakistán

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Espectrometría Raman / Azufre / Tiofenos / Compuestos de Bifenilo / Actinobacteria Límite: Humans Idioma: En Revista: Spectrochim Acta A Mol Biomol Spectrosc Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Pakistán