Hypothetical protein MAA_07646 is required for stress resistance and pathogenicity in Metarhizium robertsii.
World J Microbiol Biotechnol
; 40(5): 141, 2024 Mar 23.
Article
en En
| MEDLINE
| ID: mdl-38519797
ABSTRACT
Metarhizium robertsii, a vital entomopathogenic fungus for pest management, relies on various virulence-related proteins for infection. Identifying these proteins, especially those with unknown functions, can illuminate the fungus's virulence mechanisms. Through RNA-seq, we discovered that the hypothetical protein MAA_07646 was significantly upregulated during appressorium formation in M. robertsii. In this study, we characterized MAA_07646, finding its presence in both the nucleus and cytoplasm. Surprisingly, it did not affect vegetative growth, conidiation, or chemical tolerance. However, it played a role in heat and UV radiation sensitivity. Notably, ΔMAA_07646 exhibited reduced virulence in Galleria mellonella larvae due to impaired appressorium formation and decreased expression of virulence-related genes. In conclusion, MAA_07646 contributes to thermotolerance, UV resistance, and virulence in M. robertsii. Understanding its function sheds light on the insecticidal potential of M. robertsii's hypothetical proteins.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Metarhizium
/
Mariposas Nocturnas
Límite:
Animals
Idioma:
En
Revista:
World J Microbiol Biotechnol
Año:
2024
Tipo del documento:
Article
País de afiliación:
China